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Tested Applications
| Positive WB detected in | PC-3 cells, U-251 cells |
| Positive IHC detected in | human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
Product Information
12210-1-AP targets SH3BGRL3 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SH3BGRL3 fusion protein Ag2857 Predict reactive species |
| Full Name | SH3 domain binding glutamic acid-rich protein like 3 |
| Calculated Molecular Weight | 93 aa, 10 kDa |
| Observed Molecular Weight | 10 kDa |
| GenBank Accession Number | BC030135 |
| Gene Symbol | SH3BGRL3 |
| Gene ID (NCBI) | 83442 |
| RRID | AB_10694666 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H299 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SH3BGRL3, SH3 domain-binding glutamic acid-rich-like protein, could act as a modulator of glutaredoxin biological activity. It may play a role in cytoskeleton organization (PMID: 34380438). SH3BGRL3 acts as a potential prognostic biomarker for urothelial carcinoma and a novel binding partner of epidermal growth factor receptor (PMID: 26286913).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SH3BGRL3 antibody 12210-1-AP | Download protocol |
| IHC protocol for SH3BGRL3 antibody 12210-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
