Filter: AllWBIHCIF/ICC

WB analysis of HT-1080 using 21499-1-AP
HT-1080 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of Y79 using 21499-1-AP
Y79 cells were subjected to SDS PAGE followed by western blot with 21499-1-AP (SH3D19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human heart using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded human heart using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).
IHC staining of mouse kidney using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse heart using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21499-1-AP (SH3D19 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human kidney using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human kidney using 21499-1-AP
Immunohistochemical analysis of paraffin-embedded human kidney using 21499-1-AP (SH3D19 antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 21499-1-AP
Immunofluorescent analysis of (4% PFA) fixed A431 cells using SH3D19 antibody (21499-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).
IF Staining of HeLa using 21499-1-AP
Immunofluorescent analysis of Hela cells, using SH3D19 antibody 21499-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

SH3D19 Polyclonal antibody

SH3D19 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21499-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

ADAM binding protein Eve 1, ADAM-binding protein Eve-1, EBP, EEN binding protein, EEN-binding protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	HT-1080 cells, Y79 cells
Positive IHC detected in	mouse heart tissue, human heart tissue, human kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunohistochemistry (IHC)	IHC : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21499-1-AP targets SH3D19 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15915 Product name: Recombinant human SH3D19 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 415-764 aa of BC108890 Sequence: VLVMLKQTENNYLECQKGEDTGRVHLSQMKIITPLDEHLRSRPNDPSHAQKPVDSGAPHAVVLHDFPAEQVDDLNLTSGEIVYLLEKIDTDWYRGNCRNQIGIFPANYVKVIIDIPEGGNGKRECVSSHCVKGSRCVARFEYIGEQKDELSFSEGEIIILKEYVNEEWARGEVRGRTGIFPLNFVEPVEDYPTSGANVLSTKVPLKTKKEDSGSNSQVNSLPAEWCEALHSFTAETSDDLSFKRGDRIQILERLDSDWCRGRLQDREGIFPAVFVRPCPAEAKSMLAIVPKGRKAKALYDFRGENEDELSFKAGDIITELESVDDDWMSGELMGKSGIFPKNYIQFLQIS Predict reactive species
Full Name	SH3 domain containing 19
Calculated Molecular Weight	790 aa, 87 kDa
Observed Molecular Weight	45-47 kDa
GenBank Accession Number	BC108890
Gene Symbol	SH3D19
Gene ID (NCBI)	152503
RRID	AB_10733218
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5HYK7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SH3D19, also known as EBP, Eve-1, encodes a multiple SH3 domain-containing protein. SH3D19 plays a role in regulating A disintegrin and metalloproteases (ADAMs) in the signaling of EGFR-ligand shedding. SH3D19 is involved in the suppression of Ras-induced cellular transformation and Ras-mediated activation of ELK1(PMID: 14551139). SH3D19 is abundantly expressed in skeletal muscle and heart(PMID: 15280379).

Protocols

Product Specific Protocols
IF protocol for SH3D19 antibody 21499-1-AP	Download protocol
IHC protocol for SH3D19 antibody 21499-1-AP	Download protocol
WB protocol for SH3D19 antibody 21499-1-AP	Download protocol