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SHPRH Polyclonal antibody

SHPRH Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC

Conjugate

Unconjugated

Cat no : 21955-1-AP

Synonyms

KIAA2023, SHPRH

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse testis tissue using SHPRH Polyclonal antibody (21955-1-AP)

    WB analysis of mouse testis using 21955-1-AP

    mouse testis tissue were subjected to SDS PAGE followed by western blot with 21955-1-AP (SHPRH Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse testis tissue using SHPRH Polyclonal antibody (21955-1-AP)

    IP experiment of mouse testis using 21955-1-AP

    IP result of anti-SHPRH (IP:21955-1-AP, 4ug; Detection:21955-1-AP 1:300) with mouse testis tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon tissue using SHPRH Polyclonal antibody (21955-1-AP)

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using SHPRH Polyclonal antibody (21955-1-AP)

    IHC staining of human colon using 21955-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 21955-1-AP (SHPRH antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inmouse testis tissue
Positive IP detected inmouse testis tissue
Positive IHC detected inhuman colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21955-1-AP is SHPRH Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SHPRH fusion protein Ag16635
Full Name SNF2 histone linker PHD RING helicase
Calculated Molecular Weight 1683 aa, 193 kDa
Observed Molecular Weight 200 kDa
GenBank Accession NumberBC117686
Gene Symbol SHPRH
Gene ID (NCBI) 257218
RRIDAB_2878954
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for SHPRH antibody 21955-1-APDownload protocol
IHC protocol for SHPRH antibody 21955-1-APDownload protocol
IP protocol for SHPRH antibody 21955-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols