Tested Applications
| Positive WB detected in | THP-1 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13230-1-AP targets SIGLEC5 in WB, IHC, IF, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag4101 Product name: Recombinant human SIGLEC5 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 241-442 aa of BC029896 Sequence: IFRNGIALEILQNTSYLPVLEGQALRLLCDAPSNPPAHLSWFQGSPALNATPISNTGILELRRVRSAEEGGFTCRAQHPLGFLQIFLNLSVYSLPQLLGPSCSWEAEGLHCRCSFRARPAPSLCWRLEEKPLEGNSSQGSFKVNSSSAGPWANSSLILHGGLSSDLKVSCKAWNIYGSQSGSVLLLQGRSNLGTGVVPAALG Predict reactive species |
| Full Name | sialic acid binding Ig-like lectin 5 |
| Calculated Molecular Weight | 551 aa, 61 kDa |
| Observed Molecular Weight | 70 kDa |
| GenBank Accession Number | BC029896 |
| Gene Symbol | SIGLEC5 |
| Gene ID (NCBI) | 8778 |
| RRID | AB_10643385 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15389 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Sialic acid binding Ig-like lectin 5 (Siglec-5), also known as CD170, is a member of the CD33-related Siglec subfamily. Siglec-5 is a type-I transmembrane protein consisting of an N-terminal extracellular region that contains four extracellular immunoglobulin-like domains, a transmembrane region, and an intracellular domain with two tyrosine-based motifs (PMID: 9731071). It is expressed on neutrophils, monocytes, dendritic cells, and subsets of tissue macrophages (PMID: 15769739). Siglec-5 binds alpha-2,3-linked, alpha-2,6-linked sialic acid, and is involved in cell adhesion (PMID: 18022638).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SIGLEC5 antibody 13230-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Cell Death Dis METTL14 contributes to acute lung injury by stabilizing NLRP3 expression in an IGF2BP2-dependent manner | ||
