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SLC25A13 Polyclonal antibody

SLC25A13 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 10789-1-AP

Synonyms

ARALAR2, CITRIN, CTLN2, SLC25A13

Validation Data Gallery

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  • Western Blot (WB) analysis of mouse kidney tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    WB analysis of mouse kidney using 10789-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Raji cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    WB analysis of Raji using 10789-1-AP

    Raji cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of A431 cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    WB analysis of A431 using 10789-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    WB analysis of mouse liver using 10789-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    WB analysis of HEK-293 using 10789-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 10789-1-AP (SLC25A13 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse kidney tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IP experiment of mouse kidney using 10789-1-AP

    IP result of anti-SLC25A13 (IP:10789-1-AP, 4ug; Detection:10789-1-AP 1:1000) with mouse kidney tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IHC staining of human colon cancer using 10789-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using SLC25A13 Polyclonal antibody (10789-1-AP)

    IHC staining of human colon cancer using 10789-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10789-1-AP (SLC25A13 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of U2OS cells using SLC25A13 Polyclonal antibody (10789-1-AP)

    IF Staining of U2OS using 10789-1-AP

    Immunofluorescent analysis of (4% PFA) fixed U2OS cells using SLC25A13 antibody (10789-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

Tested Applications

Positive WB detected inmouse kidney tissue, Raji cells, A431 cells, HEK-293 cells, mouse liver tissue
Positive IP detected inmouse kidney tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2400
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10789-1-AP targets SLC25A13 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SLC25A13 fusion protein Ag1242
Full Name solute carrier family 25, member 13 (citrin)
Calculated Molecular Weight 74 kDa
Observed Molecular Weight 60-63 kDa
GenBank Accession NumberBC006566
Gene Symbol SLC25A13
Gene ID (NCBI) 10165
RRIDAB_2189738
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SLC25A13, also known as citrin, has been identified as a mitochondrial aspartate-glutamate carrier protein and may be implicated in metabolic compartmentation. It consists of 675 amino acid residues and harbors four EF-hands and six mitochondrial transmembranous (TM) spanners. Mutations in SLC25A13 gene cause adult-onset type II citrullinemia (CTLN2) and neonatal intrahepatic cholestasis (NICCD). The complete deletion or cleaved SLA25A13 proteins have been reported in patients with SLC25A13 mutation.

Protocols

Product Specific Protocols
WB protocol for SLC25A13 antibody 10789-1-APDownload protocol
IHC protocol for SLC25A13 antibody 10789-1-APDownload protocol
IF protocol for SLC25A13 antibody 10789-1-APDownload protocol
IP protocol for SLC25A13 antibody 10789-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols