Tested Applications
| Positive WB detected in | COLO 320 cells |
| Positive IHC detected in | human hysteromyoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 5 publications below |
| IHC | See 2 publications below |
Product Information
23567-1-AP targets SMTN in WB, IHC, ELISA applications and shows reactivity with mouse, human, rat samples.
| Tested Reactivity | mouse, human, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag19983 Product name: Recombinant human SMTN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 458-811 aa of BC034237 Sequence: KTTFTIEIKDGRGQASTGRVLLPTGNQRAELTLGLRAPPTLLSTSSGGKSTITRVNSPGTLARLGSVTHVTSFSHAPPSSRGGCSIKMEPEPAEPLAAAVEAANGAEQTRVNKAPEGRSPLSAEELMTIEDEGVLDKMLDQSTDFEERKLIRAALRELRQRKRDQRDKERERRLQEARGRPGEGRGNTATETTTRHSQRAADGSAVSTVTKTERLVHSNDGTRTARTTTVESSFVRRSENGSGSTMMQTKTFSSSSSSKKMGSIFDREDQASPRAGSLAALEKRQAEKKKELMKAQSLPKTSASQARKAMIEKLEKEGAAGSPGGPRAAVQRSTSFGVPNANSIKQMLLDWCRA Predict reactive species |
| Full Name | smoothelin |
| Calculated Molecular Weight | 940 aa, 102 kDa |
| Observed Molecular Weight | 117 kDa |
| GenBank Accession Number | BC034237 |
| Gene Symbol | SMTN |
| Gene ID (NCBI) | 6525 |
| RRID | AB_2879298 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen Affinity purified |
| UNIPROT ID | P53814 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SMTN, also named as Smoothelin, is a 917 amino acid protein, which contains 1 CH (calponin-homology) domain and belongs to the smoothelin family. SMTN as structural protein of the cytoskeleton involve in muscle organ development and smooth muscle contraction.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SMTN antibody 23567-1-AP | Download protocol |
| WB protocol for SMTN antibody 23567-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
bioRxiv Coordination between cytoskeletal organization, cell contraction and extracellular matrix development, is depended on LOX for aneurysm prevention | ||
FEBS J Coordination among cytoskeletal organization, cell contraction, and extracellular matrix development is dependent on LOX for aneurysm prevention | ||
Int J Nanomedicine Glycosylation-Engineered Platelet Membrane-Coated Interleukin 10 Nanoparticles for Targeted Inhibition of Vascular Restenosis | ||
J Mol Med (Berl) Vav2 promotes ductus arteriosus anatomic closure via the remodeling of smooth muscle cells by Rac1 activation |
