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  • KD/KO Validated

SMYD3 Polyclonal antibody

SMYD3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 12011-1-AP

Synonyms

SMYD3, ZMYND1, ZNFN3A1



Tested Applications

Positive WB detected inmouse brain tissue, COLO 320 cells, HeLa cells, HepG2 cells
Positive IP detected inCOLO 320 cells
Positive IHC detected inhuman breast cancer tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12011-1-AP targets SMYD3 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SMYD3 fusion protein Ag2624
Full Name SET and MYND domain containing 3
Calculated Molecular Weight428aa,49 kDa; 369aa,42 kDa
Observed Molecular Weight 42-45 kDa
GenBank Accession NumberBC031010
Gene Symbol SMYD3
Gene ID (NCBI) 64754
RRIDAB_2193981
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SMYD3,also name as ZMYND1 and ZNFN3A1, belongs to the histone-lysine methyltransferase family.It is a histone methyltransferase that plays an important role in transcriptional regulation in human carcinogenesis. It can specifically methylate histone H3 at lysine 4 and activate the transcription of a set of downstream genes, including several oncogenes (e.g., N-myc, CrkL, Wnt10b, RIZ and hTERT) and genes involved in the control of cell cycle.(PMID: 20957523). It plays an important role in transcriptional activation as a member of an RNA polymerase complex. SMYD3 is frequently overexpressed in different types of cancer cells. It functions as a coactivator of Era and potentiates Era activity in response to ligand. SMYD3 as a new coactivator for ER-mediated transcription, providing a possible link between SMYD3 overexpression and breast cancer. (PMID: 19509295) The common variable number of tandem repeats polymorphism in SMYD3 is a susceptibility factor for some types of human cancer. (PMID:16155568 ) . SMYD3 exists some isoforms with MV 49, 43 and 30 kDa.

Protocols

Product Specific Protocols
WB protocol for SMYD3 antibody 12011-1-APDownload protocol
IHC protocol for SMYD3 antibody 12011-1-APDownload protocol
IF protocol for SMYD3 antibody 12011-1-APDownload protocol
IP protocol for SMYD3 antibody 12011-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Acta Pharm Sin B

SMYD3-PARP16 axis accelerates unfolded protein response and mediates neointima formation.

Authors - Fen Long
  • KD Validated
humanWB

Carcinogenesis

miR-124 and miR-203 are epigenetically silenced tumor-suppressive microRNAs in hepatocellular carcinoma.

Authors - Furuta Mayuko M
humanWB

Int J Androl

Histone methylation is a critical regulator of the abnormal expression of POU5F1 and RASSF1A in testis cancer cell lines.

Authors - Lambrot R R
humanWB

Med Oncol

PMID: 20957523

Authors - Ren Tian-nian TN
  • KD Validated
ratWB

Cell Physiol Biochem

iTRAQ-Based Proteomic Analysis of Neonatal Kidney from Offspring of Protein Restricted Rats Reveals Abnormalities in Intraflagellar Transport Proteins.

Authors - Xiaomei Liu
humanWB,IHC

Oncogenesis

SMYD3 promotes endometrial cancer through epigenetic regulation of LIG4/XRCC4/XLF complex in non-homologous end joining repair

Authors - Yujia Huang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Deyong (Verified Customer) (03-11-2022)

It worked well in the HEK293T cells with human SMYD3 overexpression.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HEK293T cells