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Tested Applications
| Positive WB detected in | Jurkat cells, K-562 cells |
| Positive IHC detected in | human cervical cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 2 publications below |
| WB | See 3 publications below |
| IHC | See 4 publications below |
| IP | See 2 publications below |
Product Information
16807-1-AP targets SNRPB in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | SNRPB fusion protein Ag10298 Predict reactive species |
| Full Name | small nuclear ribonucleoprotein polypeptides B and B1 |
| Calculated Molecular Weight | 234aa,25 kDa; 285aa,30 kDa |
| Observed Molecular Weight | 25-30 kDa |
| GenBank Accession Number | BC003530 |
| Gene Symbol | SNRPB |
| Gene ID (NCBI) | 6628 |
| RRID | AB_2878319 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P14678 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SNRPB (Small Nuclear Ribonucleoprotein Polypeptides B and B1) is a core component of the spliceosome, a complex molecular machine that plays a critical role in pre-mRNA splicing. This process is essential for the removal of introns and the joining of exons to produce mature mRNA molecules.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for SNRPB antibody 16807-1-AP | Download protocol |
| IHC protocol for SNRPB antibody 16807-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife Type I and II PRMTs inversely regulate post-transcriptional intron detention through Sm and CHTOP methylation. | ||
Cell Death Dis SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.
| ||
Oncogene The splicing factor SNRPB promotes ovarian cancer progression through regulating aberrant exon skipping of POLA1 and BRCA2
| ||
Aging (Albany NY) SNRPB promotes the progression of hepatocellular carcinoma via regulating cell cycle, oxidative stress, and ferroptosis | ||
Ann Med SNRPB and CEP290, predicting the prognosis of diffuse large B cell lymphoma and associated with tumour immune microenvironment | ||
Adv Sci (Weinh) PRMT5 Maintains Homeostasis of the Intestinal Epithelium by Modulating Cell Proliferation and Survival |
