Featured Product
KD/KO Validated

SNX18 Polyclonal antibody

SNX18 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21946-1-AP

Publications (4)

Review Product

Download datasheet

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA, IB

Synonyms

SH3 and PX domain-containing protein 3B, SH3PXD3B, Sorting nexin-18

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

- +

Request bulk or custom quote

Proteintech Guarantee

Filter: AllWBIHC

WB analysis of MCF-7 using 21946-1-AP
WB result of SNX18 antibody (21946-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-SNX18 transfected MCF-7 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 21946-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 21946-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 21946-1-AP (SNX18 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human placenta using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat kidney using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse bladder using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded mouse bladder tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse kidney using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of rat bladder using 21946-1-AP
Immunohistochemical analysis of paraffin-embedded rat bladder tissue slide using 21946-1-AP (SNX18 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Able AI

What's the best dilution for SNX18 in ?

Overview

Tested Applications
Product Information
Publications (4)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	MCF-7 cells, MDA-MB-231 cells
Positive IHC detected in	human placenta tissue, mouse kidney tissue, mouse bladder tissue, rat bladder tissue, rat kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:16000
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21946-1-AP targets SNX18 in WB, IHC, ELISA, IB applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag16645 Product name: Recombinant human SNX18 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 55-284 aa of BC060791 Sequence: YVQVIRAPEPGPAGDGGPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQLYGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSVPPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTIDDPT Predict reactive species
Full Name	sorting nexin 18
Calculated Molecular Weight	628 aa, 69 kDa
Observed Molecular Weight	70 kDa
GenBank Accession Number	BC060791
Gene Symbol	SNX18
Gene ID (NCBI)	112574
RRID	AB_11182387
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q96RF0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SNXs (sorting nexins) are a diverse group of cytoplasmic and membrane-associated proteins that are classified by the presence of a phospholipid-binding motif-the PX domain (PMID:12461558). SNXs are involved in endocytosis and protein trafficking. SNX18, together with SNX9 and SNX33, constitutes a separate SNX9 subfamily which is required for mitosis through endocytosis processes (PMID: 22718350, 20427313). SNX18 acts as a positive regulator of autophagy and regulates ATG9A trafficking from recycling endosomes (PMID: 24113029, 29437695).

Protocols

Product Specific Protocols
IHC protocol for SNX18 antibody 21946-1-AP	Download protocol
WB protocol for SNX18 antibody 21946-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The SNX18 Polyclonal antibody (Cat# 21946-1-AP) has 4 total citations published in Advanced Science, Cell Chemical Biology, Communications Biology, and bioRxiv. The associated research fields include endocytic regulation of p53 function, multi-protein targeted degradation via the autophagy-lysosome pathway, β-arrestin-independent GPCR endocytosis mediated by the SNX9 family, and interactome mapping in Alzheimer's disease-related excitatory neurons.

Species	Application	Title
human	IB	Adv Sci (Weinh) Endocytic Control of Cell-Autonomous and Non-Cell-Autonomous Functions of p53. Authors - Roberta Cacciatore View Article
human	WB	Cell Chem Biol A nature-inspired nanoplatform for multi-protein targeted degradation via the autophagy-lysosome pathway. Authors - Bide Tong View Article
human	WB	Commun Biol SNX9 family mediates βarrestin-independent GPCR endocytosis Authors - Valeria L Robleto View Article
human	WB	bioRxiv Interactome mapping in human excitatory neurons reveals novel risk genes and pathways in Alzheimer's disease. Authors - Xiaomu Wei View Article