SOD3 Polyclonal antibody

SOD3 Polyclonal Antibody for IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IP, ELISA

Conjugate

Unconjugated

Cat no : 14316-1-AP

Synonyms

EC SOD, Extracellular superoxide dismutase, SOD3, Superoxide Dismutase 3



Tested Applications

Positive WB detected inhuman urine sample, human heart tissue, human urine, human placenta tissue
Positive IP detected inhuman placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14316-1-AP targets SOD3 in WB, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SOD3 fusion protein Ag5556
Full Name superoxide dismutase 3, extracellular
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 25-33 kDa
GenBank Accession NumberBC014418
Gene Symbol SOD3
Gene ID (NCBI) 6649
RRIDAB_10898170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SOD is located intracellular (SOD-1; cytosolic, SOD-2; mitochondrial matrix) and extracellular (SOD-3), and catalyzes the dismutation of superoxide anion to H2O2.SOD3, or EC-SOD, is the most recently characterized SOD, exists as a copper and zinc-containing tetramer, and is synthesized containing a signal peptide that directs this enzyme exclusively to extracellular spaces. SOD-3 is a Mn-containing tetrameric protein with a subunit Mr of 24-35 kDa. The activity of EC-SOD is dependent on its ability to form a tetramer of 135 kDa.

Protocols

Product Specific Protocols
WB protocol for SOD3 antibody 14316-1-APDownload protocol
IP protocol for SOD3 antibody 14316-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse,humanWB

Br J Pharmacol

Inhibition of DNA methylation derepresses PPARγ and attenuates pulmonary fibrosis.

Authors - Ai Wei
humanWB

Transl Pediatr

Identification and evaluation of fructose-bisphosphate aldolase B as a potential diagnostic biomarker in choledochal cysts patients: a quantitative proteomic analysis.

Authors - Gedong Ming
human,ratWB

Toxicol Appl Pharmacol

(-)-Epicatechin ameliorates cigarette smoke-induced lung inflammation via inhibiting ROS/NLRP3 inflammasome pathway in rats with COPD.

Authors - Xue Tian
humanWB

Clin Immunol

Oxidative stress is involved in immunosuppression and macrophage regulation in glioblastoma

Authors - Xisong Liang
  • KD Validated