Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, mouse brain tissue |
| Positive IF/ICC detected in | HaCaT cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
55063-1-AP targets SORCS2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | sortilin-related VPS10 domain containing receptor 2 |
| Calculated Molecular Weight | 128 kDa |
| Observed Molecular Weight | 128-140 kDa |
| GenBank Accession Number | NM_020777 |
| Gene Symbol | SORCS2 |
| Gene ID (NCBI) | 57537 |
| RRID | AB_10859831 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q96PQ0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SORCS2, also named as KIAA1329, is one of the VPS10 domain-containing receptors. It contains 6 BNR repeats and 1 PKD domain.. The antibody is specific to SORCS2.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SORCS2 antibody 55063-1-AP | Download protocol |
| WB protocol for SORCS2 antibody 55063-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The SORCS2 Polyclonal antibody (Cat# 55063-1-AP) has 2 total citations. These appear in PLoS One and Cytojournal. The cited research spans reproductive medicine, examining oxidative stress-responsive genes in recurrent miscarriage using IHC in human samples, and neuroscience/ophthalmology, investigating retinal ischemia-reperfusion injury and microglial activation via WB in rat models.
| Species | Application | Title |
|---|---|---|
PLoS One Identification of oxidative stress-responsive genes in recurrent miscarriage and their role in disease pathogenesis. | ||
Cytojournal Astragalus polysaccharide attenuates retinal ischemia reperfusion-induced microglial activation through sortilin-related vacuolar protein sorting 10 domain containing receptor 2/laminin subunit alpha 1 upregulation |
Reviews
What customers say
The sole review for this product is negative (1/5 stars), posted by a researcher in November 2018. The user tested the antibody on postnatal mouse cerebellum tissue (P2–P14) for both Western blot and immunohistochemistry. Despite trying multiple lysis buffers and blocking conditions (5% milk or 2% BSA), the antibody failed to produce results at dilutions of 1:100 to 1:1000. No positive outcome was reported for either application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Juan Pablo (Verified Customer) (11-29-2018) | I tried with different lysis buffer. I tried blocking with 5% milk or 2% BSA. Never worked for me. Didn't work for immunohistochemistry either dilution 1:1000 and 1:100.
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