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SORCS2 Polyclonal antibody

SORCS2 Polyclonal Antibody for WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB,ELISA

Conjugate

Unconjugated

Cat no : 55063-1-AP

Synonyms

KIAA1329, SORCS2

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using SORCS2 Polyclonal antibody (55063-1-AP)

    WB analysis of Jurkat using 55063-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SORCS2 Polyclonal antibody (55063-1-AP)

    WB analysis of mouse brain using 55063-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293 cells using SORCS2 Polyclonal antibody (55063-1-AP)

    WB analysis of HEK-293 using 55063-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 55063-1-AP (SORCS2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

Tested Applications

Positive WB detected inJurkat cells, HEK-293 cells, mouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55063-1-AP targets SORCS2 in WB,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide
Full Name sortilin-related VPS10 domain containing receptor 2
Calculated Molecular Weight 128 kDa
Observed Molecular Weight 128-140 kDa
GenBank Accession NumberNM_020777
Gene Symbol SORCS2
Gene ID (NCBI) 57537
RRIDAB_10859831
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SORCS2, also named as KIAA1329, is one of the VPS10 domain-containing receptors. It contains 6 BNR repeats and 1 PKD domain.. The antibody is specific to SORCS2.

Protocols

Product Specific Protocols
WB protocol for SORCS2 antibody 55063-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Juan Pablo (Verified Customer) (11-29-2018)

I tried with different lysis buffer. I tried blocking with 5% milk or 2% BSA. Never worked for me. Didn't work for immunohistochemistry either dilution 1:1000 and 1:100.

  • Applications: Western Blot, Immunohistochemistry,
  • Primary Antibody Dilution: 1-500
  • Cell Tissue Type: Postnatal cerebellum, from P2 to P14
SORCS2 Antibody Western Blot,Immunohistochemistry, validation (1-500 dilution) in Postnatal cerebellum, from P2 to P14 (Cat no:55063-1-AP)