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SP6 Polyclonal antibody

SP6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 21234-1-AP

Synonyms

EPFN, EPIPROFIN, SP6, Sp6 transcription factor, Transcription factor Sp6

Validation Data Gallery

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  • Western Blot (WB) analysis of fetal human brain tissue using SP6 Polyclonal antibody (21234-1-AP)

    WB analysis of fetal human brain using 21234-1-AP

    fetal human brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse brain tissue using SP6 Polyclonal antibody (21234-1-AP)

    WB analysis of mouse brain using 21234-1-AP

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse liver tissue using SP6 Polyclonal antibody (21234-1-AP)

    WB analysis of mouse liver using 21234-1-AP

    mouse liver tissue were subjected to SDS PAGE followed by western blot with 21234-1-AP (KLF14 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skin tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human skin using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skin tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human skin using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human skin using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human placenta using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human placenta tissue using SP6 Polyclonal antibody (21234-1-AP)

    IHC staining of human placenta using 21234-1-AP

    Immunohistochemical analysis of paraffin-embedded human placenta using 21234-1-AP (KLF14 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of Caco-2 cells using SP6 Polyclonal antibody (21234-1-AP)

    IF Staining of Caco-2 using 21234-1-AP

    Immunofluorescent analysis of (4% PFA) fixed Caco-2 cells using SP6 antibody (21234-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected infetal human brain tissue, mouse brain tissue
Positive IHC detected inhuman skin tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inCaco-2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21234-1-AP targets SP6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SP6 fusion protein Ag15707
Full Name Sp6 transcription factor
Calculated Molecular Weight 376 aa, 40 kDa
GenBank Accession NumberBC103951
Gene Symbol SP6
Gene ID (NCBI) 80320
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SP6 (chromosome 17) is a 376 amino acid protein, which localizes in nucleus. SP6 is a transcription factor of the SP/KLF family and an indispensable regulator of the morphological dynamics of ameloblast differentiation during tooth development. The levels of sp6 protein expressed in various tissues in the embryo. This antibody detects the SP6 protein. It can't detect the KLF14/BTEB5.

This antibody was previously listed as a KLF14 antibody, because the SP6 gene on chromosome 17q21.32 has been referred to as KLF14 in the literature. SP6 should not be confused with the KLF14 gene on chromosome 7q32.2.

Protocols

Product Specific Protocols
WB protocol for SP6 antibody 21234-1-APDownload protocol
IHC protocol for SP6 antibody 21234-1-APDownload protocol
IF protocol for SP6 antibody 21234-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Int J Biol Sci

The KLF14 Transcription Factor Regulates Glycolysis by Downregulating LDHB in Colorectal Cancer.

Authors - Guiyang Wu
View Article