Tested Applications
| Positive WB detected in | mouse testis tissue, mouse ovary tissue |
| Positive IP detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11849-1-AP targets SPATA6 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2413 Product name: Recombinant human SPATA6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 167-472 aa of BC020660 Sequence: YHLAPVEKSHGRLQNRTSRSQKKKSKSPERSKYCINAKNYEQPTISSKSHSPSPYTKRRMCELSEDTRRRLAHLNLGPYEFKKETDKPPFVIRHVDPPSPRADTLLGSSGRDCERDGWSRVHNDHSHLGCCRPKDYKVIRTPHGRDFDDSLEKCEEYLSPRSCSKPRHSARTLLVHSAPSTMPKHSPSPVLNRASLRERFHSDWCSPSNCDEIHDRDERDLEKDDELELKRSLLCRDSAYDSDPEYSSCQQPRGTFHLDDGEYWSNRAASYKGKSHRPIFENSMDKMYRNLYKKACSSASHTQESF Predict reactive species |
| Full Name | spermatogenesis associated 6 |
| Calculated Molecular Weight | 472 aa, 54 kDa |
| Observed Molecular Weight | 54-60 kDa |
| GenBank Accession Number | BC020660 |
| Gene Symbol | SPATA6 |
| Gene ID (NCBI) | 54558 |
| RRID | AB_11232594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NWH7 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Spermatogenesis-associated protein 6 (SPATA6) is a testis-enriched protein crucial for spermatogenesis and male fertility. It localizes to the sperm head-tail coupling apparatus (HTCA) and is crucial for the proper assembly of the sperm connecting piece and tight head-tail conjunction. SPATA6 is a core structural component of the sperm fibrous sheath (FS), a cytoskeletal structure in the principal piece of the sperm flagellum essential for normal sperm motility. Mutations in SPATA6 can lead to acephalic spermatozoa and male infertility (PMID: 38454159, PMID: 41058548).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| IP protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| WB protocol for SPATA6 antibody 11849-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Toxicol Appl Pharmacol Low dose lead exposure at the onset of puberty disrupts spermatogenesis-related gene expression and causes abnormal spermatogenesis in mouse. | ||
Theriogenology Effects of SPATA6 on proliferation, apoptosis and steroidogenesis of Hu sheep Leydig cells in vitro. | ||
Elife CCDC113 stabilizes sperm axoneme and head-tail coupling apparatus to ensure male fertility | ||
Clin Genet Identification of nonfunctional SPATA20 causing acephalic spermatozoa syndrome in humans |
