Tested Applications
| Positive WB detected in | HeLa cells, human colon tissue, mouse stomach tissue, rat colon tissue, mouse spleen tissue, mouse colon tissue, Jurkat cells |
| Positive IP detected in | HeLa cells, mouse colon tissue |
| Positive IHC detected in | human skin cancer tissue, human stomach cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
Product Information
14872-1-AP targets SPCS2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag6640 Product name: Recombinant human SPCS2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-226 aa of BC064957 Sequence: MAAAAVQGGRSGGSGGCSGAGGASNCGTGSGRSGLLDKWKIDDKPVKIDKWDGSAVKNSLDDSVKKVLLEKYKYVENFGLIDGRLTICTISCFFAIVALIWDYMHPFPESKPVLALCVISYFVMMGILTIYTSYKEKSIFLVAHRKDPTGMDPDDIWQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYEPEISRLHDSLAIERKIK Predict reactive species |
| Full Name | signal peptidase complex subunit 2 homolog (S. cerevisiae) |
| Calculated Molecular Weight | 25 kDa |
| Observed Molecular Weight | 25 kDa |
| GenBank Accession Number | BC064957 |
| Gene Symbol | SPCS2 |
| Gene ID (NCBI) | 9789 |
| RRID | AB_2255321 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q15005 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Signal peptidase complex subunit 2 (SPCS2) is multi-pass membrane protein as a component of the microsomal signal peptidase complex, which function to remove signal peptides from nascent proteins before translocation into the endoplasmic reticulum lumen. This protein was evidenced as a 25 kDa protein with function not well known.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for SPCS2 antibody 14872-1-AP | Download protocol |
| IP protocol for SPCS2 antibody 14872-1-AP | Download protocol |
| WB protocol for SPCS2 antibody 14872-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nature A CRISPR screen defines a signal peptide processing pathway required by flaviviruses.
| ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Matthias (Verified Customer) (10-09-2019) | Strong band at ca. 20 kDa; fainter, most likely non specific, at ca. 25 kDa; some background, but overall decent antibody
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