Tested Applications
| Positive WB detected in | Neuro-2a cells |
| Positive IP detected in | mouse testis tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17836-1-AP targets SPDYA in WB, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12041 Product name: Recombinant human SPDYA protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-313 aa of BC093005 Sequence: MRHNQMCCETPPTVTVYVKSGSNRSHQPKKPITLKRPICKDNWQAFEKNTHNNNKSKRPKGPCLVIQRQDMTAFFKLFDDDLIQDFLWMDCCCKIADKYLLAMTFVYFKRAKFTISEHTRINFFIALYLANTVEEDEEETKYEIFPWALGKNWRKLFPNFLKLRDQLWDRIDYRAIVSRRCCEEVMAIAPTHYIWQRERSVHHSGAVRNYNRDEVQLPRGPSATPVDCSLCGKKRRYVRLGLSSSSSLSSHTAGVTEKHSQDSYNSLSMDIIGDPSQAYTGSEVVNDHQSNKGKKTNFLKKDKSMEWFTGSEE Predict reactive species |
| Full Name | speedy homolog A (Xenopus laevis) |
| Calculated Molecular Weight | 313 aa, 36 kDa |
| Observed Molecular Weight | 33 kDa |
| GenBank Accession Number | BC093005 |
| Gene Symbol | SPDYA |
| Gene ID (NCBI) | 245711 |
| RRID | AB_2878448 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5MJ70 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SPDYA, also named as SPDY1, SPY1 and RINGO A, is a cell cycle regulator which regulates the G1/S phase transition of the cell cycle by binding and activating CDK1, CDK2 and CDKN1B/KIP. It has two isoforms with MW 33 kDa and 36 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for SPDYA antibody 17836-1-AP | Download protocol |
| WB protocol for SPDYA antibody 17836-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
