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KD/KO Validated

SPHK1 Polyclonal antibody

SPHK1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 10670-1-AP

Publications(89)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment

Synonyms

Acetyltransferase SPHK1, EC:2.3.1.-, EC:2.7.1.91, SK1, Sphingosine kinase 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Proteintech Guarantee

Filter: AllWBIPIHCIF/ICC

WB analysis using 10670-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10670-1-AP (SPHK1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of L02 using 10670-1-AP
IP result of anti-SPHK1 (IP:10670-1-AP, 4ug; Detection:10670-1-AP 1:300) with L02 cells lysate 2560 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human colon cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human stomach cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse pancreas using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse stomach using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human breast cancer using 10670-1-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10670-1-AP (SPHK1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of L02 using 10670-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed L02 cells using SPHK1 antibody (10670-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (89)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HepG2 cells, L02 cells, MCF-7 cells，MDA-MB-231 cells
Positive IP detected in	L02 cells
Positive IHC detected in	human breast cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	L02 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:200-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10670-1-AP targets SPHK1 in WB, IHC, IF/ICC, IP, CoIP, ELISA, Cell treatment applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1051 Product name: Recombinant human SPHK1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 85-384 aa of BC008040 Sequence: HEVVNGLMERPDWETAIQKPLCSLPAGSGNALAASLNHYAGYEQVTNEDLLTNCTLLLCRRLLSPMNLLSLHTASGLRLFSVLSLAWGFIADVDLESEKYRRLGEMRFTLGTFLLLAALRTYRGRLAYLPVGRVGSKTPASPVVVQQGPVDAHLVPLEEPVPSHWTVVPDEDFVLVLALLHSHLGSEMFAAPMGRCAAGVMHLFYVRAGVSRAMLLRLFLAMEKGRHMEYECPYLVYVPVVAFRLEPKDGKGVFAVDGELMVSEAVQGQVHPNYFWMVSGCVEPPPSWKPQQMPPPEEPL Predict reactive species
Full Name	sphingosine kinase 1
Calculated Molecular Weight	43 kDa
Observed Molecular Weight	42 kDa
GenBank Accession Number	BC008040
Gene Symbol	SPHK1
Gene ID (NCBI)	8877
RRID	AB_2195809
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9NYA1
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SPHK(Sphingosine kinase 1) is also named as SPHK, SPK.It catalyzes the phosphorylation of sphingosine to form sphingosine 1-phosphate (SPP), a lipid mediator with both intra- and extracellular functions and is precariously perched at the balance point between progrowth and pro-death signalling in the cell.Endogenous SPHK1 purified from placenta has identical enzymatic characteristics, suggesting posttranslational modification does not effect functional properties(PMID:10947957).It has 2 isoforms produced by alternative splicing and can form the oligomeric structure(PMID:22335554).

Protocols

Product Specific Protocols
IF protocol for SPHK1 antibody 10670-1-AP	Download protocol
IHC protocol for SPHK1 antibody 10670-1-AP	Download protocol
IP protocol for SPHK1 antibody 10670-1-AP	Download protocol
WB protocol for SPHK1 antibody 10670-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Cell Death Discov SPHK1 enhances olaparib resistance in ovarian cancer through the NFκB/NRF2/ferroptosis pathway Authors - Kai Teng View Article
	WB	Food Funct The preventive effect of mussel oil on gestational diabetes mellitus in pregnant mice fed by a high-fat and high-sucrose diet Authors - Kelei Li View Article
human	WB,IP	Cells UCHL1 Regulates Radiation Lung Injury via Sphingosine Kinase-1 Authors - Yulia Epshtein View Article
human	WB	Pharmacol Res Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice. Authors - Jiale Zhu View Article
human	WB	Biochem Pharmacol An inducible sphingosine kinase 1 in hepatic stellate cells potentiates liver fibrosis Authors - Jin Sol Baek KD Validated View Article
human	IHC	Cancer Cell Int Polyamine metabolism patterns characterized tumor microenvironment, prognosis, and response to immunotherapy in colorectal cancer Authors - Enkui Zhang View Article