SQLE Antibody
Rabbit Polyclonal
Catalog number: 12544-1-AP
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Applications
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | hamster, mouse |
| Positive WB detected in | A549 cells, HEK-293 cells |
| Recommended dilution | WB: 1:200-1:2000 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
A549 cells were subjected to SDS PAGE followed by western blot with 12544-1-AP(SQLE Antibody) at dilution of 1:600
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | SQLE fusion protein ag3266 | Full name | squalene epoxidase |
|
Calculated molecular weight |
574aa,64kd |
Observed molecular
weight |
64kd |
|
GenBank accession number |
BC017033 | Gene ID (NCBI) | 6713 |
| Gene symbol | SQLE | Synonyms | FLJ30795 |
Background
SQLE, also named as ERG1, SE and SM, belongs to the squalene monooxygenase family. It catalyzes the first oxygenation step in cholesterol synthesis, acting on squalene before cyclization into the basic steroid structure. SQLE may serve as a flux-controlling enzyme beyond 3-hydroxy-3-methylglutaryl-coenzyme A reductase (HMGR, considered as rate limiting). It is also posttranslationally regulated by cholesterol-dependent proteasomal degradation.(PMID:21356516) The MW of SQLE is about 64kd. |
