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SR140 Polyclonal antibody

SR140 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 21399-1-AP
Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

U2SURP, U2-associated protein SR140, U2 snRNP-associated SURP motif-containing protein, KIAA0332, 140 kDa Ser/Arg-rich domain protein

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
CoraLite® Plus 488
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  • Western Blot (WB) analysis of mouse heart tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of mouse heart using 21399-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of HeLa using 21399-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of human brain tissue using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of human brain using 21399-1-AP

    human brain tissue were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Western Blot (WB) analysis of L02 cells using SR140 Polyclonal antibody (21399-1-AP)

    WB analysis of L02 using 21399-1-AP

    L02 cells were subjected to SDS PAGE followed by western blot with 21399-1-AP (SR140 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 10x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunohistochemistry (IHC) staining of human breast cancer tissue using SR140 Polyclonal antibody (21399-1-AP)

    IHC staining of human breast cancer using 21399-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 21399-1-AP (SR140 antibody) at dilution of 1:100 (under 40x lens).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HEK-293 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using SR140 antibody (21399-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HepG2 using 21399-1-AP

    Immunofluorescent analysis of HepG2 cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using SR140 Polyclonal antibody (21399-1-AP)

    IF Staining of HeLa using 21399-1-AP

    Immunofluorescent analysis of Hela cells, using SR140 antibody 21399-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

    This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Tested Applications

Positive WB detected inmouse heart tissue, HeLa cells, human brain tissue, L02 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells, HepG2 cells, HEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21399-1-AP targets SR140 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16093

Product name: Recombinant human SR140 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 685-1029 aa of BC111692

Sequence: KETEDVPDDLDGAPIEEELDGAPLEDVDGIPIDATPIDDLDGVPIKSLDDDLDGVPLDATEDSKKNEPIFKVAPSKWEAVDESELEAQAVTTSKWELFDQHEESEEEENQNQEEESEDEEDTQSSKSEEHHLYSNPIKEEMTESKFSKYSEMSEEKRAKLREIELKVMKFQDELESGKRPKKPGQSFQEQVEHYRDKLLQREKEKELERERERDKKDKEKLESRSKDKKEKDECTPTRKERKRRHSTSPSPSRSSSGRRVKSPSPKSERSERSERSHKESSRSRSSHKDSPRDVSKKAKRSPSGSRTPKRSRRSRSRSPKKSGKKSRSQSRSPHRSHKKSKKNKH

Predict reactive species
Full Name U2-associated SR140 protein
Calculated Molecular Weight 1029 aa, 118 kDa
Observed Molecular Weight 120-140 kDa
GenBank Accession NumberBC111692
Gene Symbol SR140
Gene ID (NCBI) 23350
RRIDAB_10863661
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO15042
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IF protocol for SR140 antibody 21399-1-APDownload protocol
IHC protocol for SR140 antibody 21399-1-APDownload protocol
WB protocol for SR140 antibody 21399-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

SpeciesApplicationTitle
humanWB

Sci Rep

Comprehensive systems biology analysis reveals splicing factor contributions to cutaneous melanoma progression

Authors - Shuting Zhu
View Article
humanWB

Biochem Biophys Res Commun

Exosomal circ_0058493 promotes imatinib resistance via miR-548b-3p/U2SURP axis in CML cells.

Authors - Bing-Jie Tang
  • KD Validated
View Article
humanWB,IHC

Immunol Res

Integrative bioinformatics and experimental analyses identify U2SURP as a novel lactylation-related prognostic signature in esophageal carcinoma

Authors - Xuan Zheng
View Article
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