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Tested Applications
| Positive WB detected in | A431 cells, MCF-7 cells, HeLa cells, HepG2 cells |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:6000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18699-1-AP targets SRA1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | steroid receptor RNA activator 1 |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | 33-35 kDa |
| GenBank Accession Number | BC156760 |
| Gene Symbol | SRA1 |
| Gene ID (NCBI) | 10011 |
| RRID | AB_2270887 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9HD15 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Steroid receptor RNA activator 1 (SRA1), also known as seroid receptor RNA activator protein (SRAP) or SRA, is involved in modulating the activity of multiple transcription factors including the estrogen receptor (ER) (PMID: 20398657). It may play a role in tumorigenesis (PMID: 16152589). The SRA1 gene encodes both SRA1 protein and a non-coding functional RNA that functions as part of a ribonucleoprotein complex activating steroid receptor induced transcription.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for SRA1 antibody 18699-1-AP | Download protocol |
| WB protocol for SRA1 antibody 18699-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Atherosclerosis Flagellar hook protein FlgE promotes macrophage activation and atherosclerosis by targeting ATP5B | ||
Transl Neurodegener Nanotechnology for microglial targeting and inhibition of neuroinflammation underlying Alzheimer's pathology |
