SRPRB Polyclonal antibody

SRPRB Polyclonal Antibody for WB, IHC, ELISA

Cat No. 14636-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Protein APMCF1, PSEC0230, Signal recognition particle receptor subunit beta, SR-beta

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

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Filter: AllWBIHC

WB analysis using 14636-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14636-1-AP (SRPRB antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of human liver using 14636-1-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 14636-1-AP (SRPRB antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 14636-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of human liver using 14636-1-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14636-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse brain using 14636-1-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14636-1-AP (SRPRB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	rat liver tissue, human liver tissue, mouse liver tissue, MCF-7 cells, HepG2 cells, COLO 320 cells
Positive IHC detected in	human liver tissue, mouse brain tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14636-1-AP targets SRPRB in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6215 Product name: Recombinant human SRPRB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-271 aa of BC065299 Sequence: MASADSRRVADGGGAGGTFQPYLDTLRQELQQTDPTLLSVVVAVLAVLLTLVFWKLIRSRRSSQRAVLLVGLCDSGKTLLFVRLLTGLYRDTQTSITDSCAVYRVNNNRGNSLTLIDLPGHESLRLQFLERFKSSARAIVFVVDSAAFQREVKDVAEFLYQVLIDSMGLKNTPSFLIACNKQDIAMAKSAKLIQQQLEKELNTLRVTRSAAPSTLDSSSTAPAQLGKKGKEFEFSQLPLKVEFLECSAKGGRGDVGSADIQDLEKWLAKIA Predict reactive species
Full Name	signal recognition particle receptor, B subunit
Calculated Molecular Weight	30 kDa
Observed Molecular Weight	30 kDa
GenBank Accession Number	BC065299
Gene Symbol	SRPRB
Gene ID (NCBI)	58477
RRID	AB_2194868
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9Y5M8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SRPRB is a signal recognition granule receptor β subunit protein, belonging to the GTPase superfamily of transmembrane GTPases. Located on the endoplasmic reticulum membrane, its main function is to anchor the signal recognition granule receptor α subunit to the endoplasmic reticulum membrane, participating in the co-translation of secretory proteins and membrane proteins targeting the endoplasmic reticulum membrane.

Protocols

Product Specific Protocols
IHC protocol for SRPRB antibody 14636-1-AP	Download protocol
WB protocol for SRPRB antibody 14636-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Gene SRPRB can regulate proliferation and migration in Triple-Negative breast cancer cells. Authors - Huan Liu KD Validated View Article
human	WB	Sci Adv Signal recognition particle receptor-β (SR-β) coordinates cotranslational N-glycosylation Authors - Chatchai Phoomak View Article