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Tested Applications
| Positive WB detected in | mouse testis tissue, rat testis tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 2 publications below |
| IF | See 3 publications below |
Product Information
11797-1-AP targets NA14 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2367 Product name: Recombinant human NA14 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-119 aa of BC000864 Sequence: MTQQGAALQNYNNELVKCIEELCQKREELCRQIQEEEDEKQRLQNEVRQLTEKLARVNENLARKIASRNEFDRTIAETEAAYLKILESSQTLLSVLKREAGNLTKATAPDQKSSGGRDS Predict reactive species |
| Full Name | Sjogren syndrome nuclear autoantigen 1 |
| Calculated Molecular Weight | 119 aa, 14 kDa |
| Observed Molecular Weight | 14 kDa |
| GenBank Accession Number | BC000864 |
| Gene Symbol | SSNA1 |
| Gene ID (NCBI) | 8636 |
| RRID | AB_2195497 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O43805 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
SSNA1, also named as NA14 and DIP13, may be an important adaptor to target spastin activity at the centrosome. SSNA1 is a 14 kDa protetin which locates the basal bodies and flagella of human sperm cells and the centrosome of cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for NA14 antibody 11797-1-AP | Download protocol |
| WB protocol for NA14 antibody 11797-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
MBio The cellular protein La functions in enhancement of virus release through lipid rafts facilitated by murine leukemia virus glycosylated Gag. | ||
Mol Biol Cell Functional characterization of putative cilia genes by high-content analysis. | ||
PLoS One Spastin-Interacting Protein NA14/SSNA1 Functions in Cytokinesis and Axon Development. | ||
Biosci Trends Identification of MIWI-associated Poly(A) RNAs by immunoprecipitation with an anti-MIWI monoclonal antibody. |
