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WB analysis of HepG2 using 17186-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of C6 using 17186-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 17186-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 17186-1-AP (STEAP3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human liver using 17186-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 10x lens).
IHC staining of human liver using 17186-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 17186-1-AP (STEAP3 antibody) at dilution of 1:100 (under 40x lens).

Featured Product
KD/KO Validated

STEAP3 Polyclonal antibody

Name: STEAP3 Polyclonal antibody
Brand: Proteintech
SKU: 17186-1-AP

STEAP3 Polyclonal Antibody for ELISA

Cat No. 17186-1-AP

Publications(17)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

dudlin 2, Dudulin 2, hpHyde, hTSAP6, Metalloreductase STEAP3, pHyde, STEAP family member 3, STEAP3, STMP3, TSAP6

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

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Overview

Tested Applications
Product Information
Publications (17)
Protocols

Print datasheet

MSDS SDS

Published Applications

KD/KO	See 1 publications below
WB	See 14 publications below
IHC	See 5 publications below
IF	See 1 publications below

Product Information

17186-1-AP targets STEAP3 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag10970 Product name: Recombinant human STEAP3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-204 aa of BC042150 Sequence: MPEEMDKPLISLHLVDSDSSLAKVPDEAPKVGILGSGDFARSLATRLVGSGFKVVVGSRNPKRTARLFPSAAQVTFQEEAVSSPEVIFVAVFREHYSSLCSLSDQLAGKILVDVSNPTEQEHLQHRESNAEYLASLFPTCTVVKAFNVISAWTLQAGPRDGNRQVPICGDQPEAKRAVSEMALAMGFMPVDMGSLASAWEVEAM Predict reactive species
Full Name	STEAP family member 3
Calculated Molecular Weight	488 aa, 55 kDa
GenBank Accession Number	BC042150
Gene Symbol	STEAP3
Gene ID (NCBI)	55240
RRID	AB_2197841
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q658P3
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for STEAP3 antibody 17186-1-AP	Download protocol
WB protocol for STEAP3 antibody 17186-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Nat Cell Biol A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport. Authors - Steinberg Florian F View Article
human	WB	Nat Commun Regulation of the endosomal SNX27-retromer by OTULIN. Authors - Aurelia Stangl View Article
mouse	WB	J Exp Med Ferrous iron-activatable drug conjugate achieves potent MAPK blockade in KRAS-driven tumors. Authors - Honglin Jiang View Article
mouse	WB	Int J Biol Macromol Exosomes from tannic acid-stimulated macrophages accelerate wound healing through miR-221-3p mediated fibroblasts migration by targeting CDKN1b Authors - Xu Song View Article
human	WB	J Nucl Med Ferronostics: Measuring Tumoral Ferrous Iron with PET to Predict Sensitivity to Iron-Targeted Cancer Therapies. Authors - Ning Zhao View Article
mouse	WB	Blood Adv Differences in Steap3 expression are a mechanism of genetic variation of RBC storage and oxidative damage in mice. Authors - Heather L Howie View Article

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WB analysis of HepG2 using 17186-1-AP

WB analysis of C6 using 17186-1-AP

WB analysis of HepG2 using 17186-1-AP

IHC staining of human liver using 17186-1-AP

IHC staining of human liver using 17186-1-AP

STEAP3 Polyclonal antibody

STEAP3 Polyclonal Antibody for ELISA

-/ -

Published Applications

Product Information

Protocols

Publications

A global analysis of SNX27-retromer assembly and cargo specificity reveals a function in glucose and metal ion transport.

Regulation of the endosomal SNX27-retromer by OTULIN.

Ferrous iron-activatable drug conjugate achieves potent MAPK blockade in KRAS-driven tumors.

Exosomes from tannic acid-stimulated macrophages accelerate wound healing through miR-221-3p mediated fibroblasts migration by targeting CDKN1b

Ferronostics: Measuring Tumoral Ferrous Iron with PET to Predict Sensitivity to Iron-Targeted Cancer Therapies.

Differences in Steap3 expression are a mechanism of genetic variation of RBC storage and oxidative damage in mice.