Tested Applications
| Positive WB detected in | pig brain tissue, HepG2 cells, MCF-7 cells, RAW 264.7 cells |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human stomach cancer tissue, human endometrial cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60052-1-Ig targets STOML2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples.
| Tested Reactivity | human, mouse, rat, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag0363 Product name: Recombinant human STOML2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 82-356 aa of BC002442 Sequence: IVINVPEQSAVTLDNVTLQIDGVLYLRIMDPYKASYGVEDPEYAVTQLAQTTMRSELGKLSLDKVFRERESLNASIVDAINQAADCWGIRCLRYEIKDIHVPPRVKESMQMQVEAERRKRATVLESEGTRESAINVAEGKKQAQILASEAEKAEQINQAAGEASAVLAKAKAKAEAIRILAAALTQHNGDAAASLTVAEQYVSAFSKLAKDSNTILLPSNPGDVTSMVAQAMGVYGALTKAPVPGTPDSLSSGSSRDVQGTDASLDEELDRVKMS Predict reactive species |
| Full Name | stomatin (EPB72)-like 2 |
| Calculated Molecular Weight | 356 aa, 39 kDa |
| Observed Molecular Weight | 39 kDa |
| GenBank Accession Number | BC002442 |
| Gene Symbol | STOML2 |
| Gene ID (NCBI) | 30968 |
| RRID | AB_2271235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9UJZ1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for STOML2 antibody 60052-1-Ig | Download protocol |
| IHC protocol for STOML2 antibody 60052-1-Ig | Download protocol |
| IP protocol for STOML2 antibody 60052-1-Ig | Download protocol |
| WB protocol for STOML2 antibody 60052-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
STOML2 (SLP-2/PHB2) Recombinant IgG Antibody (Cat# 60052-1-Ig) has 12 citations published in journals including Science, Nature Communications, Molecular Cell, Cell Death & Disease, Cell Death Discovery, Communications Biology, International Journal of Cancer, Journal of Cell Science, and Oncology Letters. Research fields encompass mitochondrial biology (division, cristae remodeling, mitophagy), oncology (pancreatic, lung, ovarian, colorectal, hepatocellular cancers), neuronal hypoxia response, and membrane proteomics.
| Species | Application | Title |
|---|---|---|
Science Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.
| ||
Mol Cell β-hydroxybutyrate facilitates mitochondrial-derived vesicle biogenesis and improves mitochondrial functions | ||
Cell Death Dis Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.
| ||
Cell Death Dis STOML2 restricts mitophagy and increases chemosensitivity in pancreatic cancer through stabilizing PARL-induced PINK1 degradation | ||
Cell Death Discov Novel mechanism of neuronal hypoxia response: HIF-1α/STOML2 mediated PINK1-dependent mitophagy activation against neuronal injury. |
