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  • Featured Product
  • KD/KO Validated

STOML2 Monoclonal antibody

STOML2 Monoclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat, pig

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

1A2E9

Cat no : 60052-1-Ig

Synonyms

EPB72 like protein 2, HSPC108, SLP 2, SLP2, stomatin (EPB72) like 2, Stomatin like protein 2, STOML2

Validation Data Gallery

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  • Western Blot (WB) analysis of pig brain tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of pig brain using 60052-1-Ig

    Pig, rat, and mouse brain tissues were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of MCF-7 using 60052-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of HepG2 using 60052-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of RAW 264.7 cells using STOML2 Monoclonal antibody (60052-1-Ig)

    WB analysis of RAW 264.7 using 60052-1-Ig

    RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60052-1-Ig (STOML2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of mouse brain tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IP experiment of mouse brain using 60052-1-Ig

    IP result of anti-STOML2 (IP:60052-1-Ig, 4ug; Detection:60052-1-Ig 1:1000) with mouse brain tissue lysate 4000ug.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human stomach cancer using 60052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human stomach cancer using 60052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 60052-1-Ig (STOML2 antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human endometrial cancer using 60052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human endometrial cancer tissue using STOML2 Monoclonal antibody (60052-1-Ig)

    IHC staining of human endometrial cancer using 60052-1-Ig

    Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 60052-1-Ig (STOML2 Antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inpig brain tissue, HepG2 cells, MCF-7 cells, RAW 264.7 cells
Positive IP detected inmouse brain tissue
Positive IHC detected inhuman stomach cancer tissue, human endometrial cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

60052-1-Ig targets STOML2 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat, pig samples.

Tested Reactivity human, mouse, rat, pig
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen STOML2 fusion protein Ag0363
Full Name stomatin (EPB72)-like 2
Calculated Molecular Weight 356 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC002442
Gene Symbol STOML2
Gene ID (NCBI) 30968
RRIDAB_2271235
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Human stomatin (band 7.2b) is a 31-kDa erythrocyte membrane protein of unknown function but implicated in the control of ion channel permeability, mechanoreception, and lipid domain organization. Stomatin (EPB72)-like 2 (STOML2, synonyms: SLP-2, HSPC108) is a 38.5-kDa protein that is overall approximately 20% similar to human stomatin. STOML2 is also present in mature human erythrocytes , but lacks a characteristic NH(2)-terminal hydrophobic domain found in other stomatin homologues. STOML2 may link stomatin or other integral membrane proteins to the peripheral cytoskeleton and thereby play a role in regulating ion channel conductances or the organization of sphingolipid and cholesterol-rich lipid rafts.

Protocols

Product Specific Protocols
WB protocol for STOML2 antibody 60052-1-IgDownload protocol
IHC protocol for STOML2 antibody 60052-1-IgDownload protocol
IP protocol for STOML2 antibody 60052-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Science

Golgi-derived PI(4)P-containing vesicles drive late steps of mitochondrial division.

Authors - Shun Nagashima
  • KD Validated
View Article
humanWB

Mol Cell Proteomics

An informatics-assisted label-free approach for personalized tissue membrane proteomics: case study on colorectal cancer.

Authors - Han Chia-Li CL
View Article
human,mouseIF,IP

Int J Mol Sci

The Mitochondrial PHB2/OMA1/DELE1 Pathway Cooperates with Endoplasmic Reticulum Stress to Facilitate the Response to Chemotherapeutics in Ovarian Cancer.

Authors - Meiyu Cheng
View Article
humanIHC

Int J Cancer

Novel prognostic protein markers of resectable pancreatic cancer identified by coupled shotgun and targeted proteomics using formalin-fixed paraffin-embedded tissues.

Authors - Takadate Tatsuyuki T
View Article
humanWB

Cell Death Dis

Stomatin-like protein 2 regulates survivin expression in non-small cell lung cancer cells through β-catenin signaling pathway.

Authors - Cheng-Ta Yang
  • KD Validated
View Article
humanWB

J Cell Sci

Deficiency of PHB complex impairs respiratory supercomplex formation and activates mitochondrial flashes.

Authors - Chongshu Jian
  • KD Validated
View Article