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WB analysis of mouse brain using 10976-4-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 10976-4-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of BxPC-3 using 10976-4-AP
BxPC-3 cells were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10976-4-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10976-4-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of human heart using 10976-4-AP
human heart tissue were subjected to SDS PAGE followed by western blot with 10976-4-AP (STXBP6 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 10976-4-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10976-4-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10976-4-AP (STXBP6 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 10976-4-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer using 10976-4-AP (STXBP6 antibody) at dilution of 1:50 (under 10x lens).

STXBP6 Polyclonal antibody

Name: STXBP6 Polyclonal antibody
Brand: Proteintech
SKU: 10976-4-AP

STXBP6 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 10976-4-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

Amisyn, Syntaxin-binding protein 6, STXBP 6, HSPC156

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, human heart tissue, human brain tissue, BxPC-3 cells, rat brain tissue
Positive IHC detected in	human lung cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunohistochemistry (IHC)	IHC : 1:50-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 1 publications below
IHC	See 1 publications below

Product Information

10976-4-AP targets STXBP6 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag1427 Product name: Recombinant human STXBP6 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-210 aa of BC009499 Sequence: MSAKSAISKEIFAPLDERMLGAVQVKRRTKKKIPFLATGGQGEYLTYICLSVTNKKPTQASITKVKQFEGSTSFVRRSQWMLEQLRQVNGIDPNGDSAEFDLLFENAFDQWVASTASEKCTFFQILHHTCQRYLTDRKPEFINCQSKIMGGNSILHSAADSVTSAVQKASQALNERGERLGRAEEKTEDLKNSAQQFAETAHKLAMKHKC Predict reactive species
Full Name	syntaxin binding protein 6 (amisyn)
Calculated Molecular Weight	25 kDa or 45 kDa
Observed Molecular Weight	28-30 kDa
GenBank Accession Number	BC009499
Gene Symbol	STXBP6
Gene ID (NCBI)	29091
RRID	AB_2197002
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NFX7
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for STXBP6 antibody 10976-4-AP	Download protocol
WB protocol for STXBP6 antibody 10976-4-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	IHC	Res Sq Novel Pathological Mechanisms Revealed by Spatial Transcriptomic Analysis of Hippocampus in Aged Control, Primary Age-Related Tauopathy, and Alzheimer's Disease. Authors - Hong-Wen Deng View Article
mouse,rat	WB	BMC Med A functional variant rs912304 for late-onset T1D risk contributes to islet dysfunction by regulating proinflammatory cytokine-responsive gene STXBP6 expression Authors - Yu Qian View Article