SULT2B1 Polyclonal antibody

SULT2B1 Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 12879-1-AP

Synonyms

Alcohol sulfotransferase, HSST2, ST2B1, Sulfotransferase 2B1, SULT2B1, sult2b1a, sult2b1b



Tested Applications

Positive WB detected inmouse skin tissue
Positive IP detected inmouse skin tissue
Positive IHC detected inhuman prostate cancer tissue, human placenta tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 2 publications below

Product Information

12879-1-AP targets SULT2B1 in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen SULT2B1 fusion protein Ag3838
Full Name sulfotransferase family, cytosolic, 2B, member 1
Calculated Molecular Weight 365 aa, 41 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC034694
Gene Symbol SULT2B1
Gene ID (NCBI) 6820
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SULT2B1 is a member of hydroxysteroid sulfotransferase (SULT) family that catalyzes sulfonation of hormones and other molecules. SULT2B1 encodes two isoforms, SULT2B1a and SULT2B1b, that differ only at their amino termini but have distinct biochemical properties as well as tissue distribution. The human fetal brain appears to only express the SULT2B1a isoform, while SULT2B1b is the predominant hydroxysteroid sulfotransferase expressed in human skin. Northern blot analysis revealed SULT2B1a has a limited tissue distribution in liver, adrenal and stomach, whereas SULT2B1b can be detected in a variety of hormone-responsive tissues including placenta, ovary, uterus, and prostate. This antibody recognizes both of ULT2B1a and SULT2B1b.

Publications

SpeciesApplicationTitle
humanWB

J Lipid Res

Activation of liver X receptor plays a central role in antiviral actions of 25-hydroxycholesterol.

Authors - Ying Liu
mouseWB

Drug Metab Dispos

Sulfotransferase 4A1 (SULT4A1) increases its expression in mouse neurons as they mature.

Authors - Takuyu Hashiguchi