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WB analysis using 12826-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 12826-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 12826-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of PC-3 using 12826-1-AP
PC-3 cells were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa cells using 12826-1-AP
HeLa cell were subjected to SDS PAGE followed by western blot with 12826-1-AP (SUPV3L1 Antibody) at dilution of 1:500.
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IP experiment of HeLa using 12826-1-AP
IP result of anti-SUPV3L1 (IP:12826-1-AP, 4ug; Detection:12826-1-AP 1:300) with HeLa cells lysate 1200ug.
IHC staining of human stomach cancer using 12826-1-AP
Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 12826-1-AP
Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 12826-1-AP (SUPV3L1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

SUPV3L1 Polyclonal antibody

Name: SUPV3L1 Polyclonal antibody
Brand: Proteintech
SKU: 12826-1-AP

SUPV3L1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 12826-1-AP

Publications(2)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

SUV3-like protein 1, SUV3 like protein 1, SUV3, Suppressor of var1 3-like protein 1, EC:3.6.4.13

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (2)
Protocols

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293 cells, PC-3 cells, HeLa cells
Positive IP detected in	HeLa cells
Positive IHC detected in	human stomach cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WB	See 2 publications below

Product Information

12826-1-AP targets SUPV3L1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag3542 Product name: Recombinant human SUPV3L1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 441-786 aa of BC036112 Sequence: YSLIKPSINEKGERELEPITTSQALQIAGRAGRFSSRFKEGEVTTMNHEDLSLLKEILKRPVDPIRAAGLHPTAEQIEMFAYHLPDATLSNLIDIFVDFSQVDGQYFVCNMDDFKFSAELIQHIPLSLRVRYVFCTAPINKKQPFVCSSLLQFARQYSRNEPLTFAWLRRYIKWPLLPPKNIKDLMDLEAVHDVLDLYLWLSYRFMDMFPDASLIRDLQKELDGIIQDGVHNITKLIKMSETHKLLNLEGFPSGSQSRLSGTLKSQARRTRGTKALGSKATEPPSPDAGELSLASRLVQQGLLTPDMLKQLEKEWMTQQTEHNKEKTESGTHPKGTRRKKKEPDSD Predict reactive species
Full Name	suppressor of var1, 3-like 1 (S. cerevisiae)
Calculated Molecular Weight	786 aa, 88 kDa
Observed Molecular Weight	70-80 kDa
GenBank Accession Number	BC036112
Gene Symbol	SUPV3L1
Gene ID (NCBI)	6832
RRID	AB_2877885
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8IYB8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

SUPV3L1 is a major helicase player in mitochondrial RNA metabolism. It is involved in the degradation of non-coding mitochondrial transcripts (MT-ncRNA) and tRNA-like molecules (PMID: 29967381).

Protocols

Product Specific Protocols
IF protocol for SUPV3L1 antibody 12826-1-AP	Download protocol
IHC protocol for SUPV3L1 antibody 12826-1-AP	Download protocol
IP protocol for SUPV3L1 antibody 12826-1-AP	Download protocol
WB protocol for SUPV3L1 antibody 12826-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	J Inherit Metab Dis Mitochondrial RNA processing defect caused by a SUPV3L1 mutation in two siblings with a novel neurodegenerative syndrome. Authors - Selma L van Esveld View Article
human	WB	Biochimie Cathepsin D inhibition during neuronal differentiation selectively affects individual proteins instead of overall protein turnover Authors - Johannes Schneider View Article