SV2C Polyclonal antibody

SV2C Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 21271-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Synaptic vesicle glycoprotein 2C

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

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Filter: AllWBIPIHC

WB analysis of mouse brain using 21271-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of C6 using 21271-1-AP
C6 cells were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of mouse skin using 21271-1-AP
mouse skin tissue were subjected to SDS PAGE followed by western blot with 21271-1-AP (SV2C antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of mouse brain using 21271-1-AP
IP result of anti-SV2C (IP:21271-1-AP, 4ug; Detection:21271-1-AP 1:500) with mouse brain tissue lysate 3440ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 21271-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IHC staining of mouse cerebellum using 21271-1-AP
Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 21271-1-AP (SV2C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	mouse brain tissue, mouse skin tissue, C6 cells
Positive IP detected in	mouse brain tissue
Positive IHC detected in	mouse cerebellum tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

21271-1-AP targets SV2C in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag15745 Product name: Recombinant human SV2C protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 450-524 aa of BC100826 Sequence: LSVWFPDVIKPLQSDEYALLTRNVERDKYANFTINFTMENQIHTGMEYDNGRFIGVKFKSVTFKDSVFKSCTFED Predict reactive species
Full Name	synaptic vesicle glycoprotein 2C
Calculated Molecular Weight	727 aa, 82 kDa
Observed Molecular Weight	80-95 kDa
GenBank Accession Number	BC100826
Gene Symbol	SV2C
Gene ID (NCBI)	22987
RRID	AB_10755297
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q496J9
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
IHC protocol for SV2C antibody 21271-1-AP	Download protocol
IP protocol for SV2C antibody 21271-1-AP	Download protocol
WB protocol for SV2C antibody 21271-1-AP	Download protocol