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WB analysis of K-562 using 11519-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of MCF-7 using 11519-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 11519-1-AP (Symplekin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of K-562 using 11519-1-AP
IP result of anti-Symplekin (IP:11519-1-AP, 4ug; Detection:11519-1-AP 1:500) with K-562 cells lysate 3600ug.
IHC staining of human testis using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human testis using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human testis using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human liver using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human colon cancer using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human colon cancer using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human colon cancer using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).
IHC staining of human placenta using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human placenta using 11519-1-AP
Immunohistochemical analysis of paraffin-embedded human placenta using 11519-1-AP (Symplekin antibody) at dilution of 1:50 (under 40x lens).

Symplekin Polyclonal antibody

Name: Symplekin Polyclonal antibody
Brand: Proteintech
SKU: 11519-1-AP
Rating: 5.00 (1 reviews)

Symplekin Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 11519-1-AP

Publications(1)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Synonyms

SPK, SYM, SYMPK, symplekin

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (1)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	K-562 cells, MCF-7 cells
Positive IP detected in	K-562 cells
Positive IHC detected in	human testis tissue, human colon cancer tissue, human liver tissue, human placenta tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 1 publications below
IF	See 1 publications below

Product Information

11519-1-AP targets Symplekin in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2081 Product name: Recombinant human SYMPK protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 118-478 aa of BC006567 Sequence: MTQLYKVALQWMVKSRVISELQEACWDMVSAMAGDIILLLDSDNDGIRTHAIKFVEGLIVTLSPRMADSEIPRRQEHDISLDRIPRDHPYIQYNVLWEEGKAALEQLLKFMVHPAISSINLTTALGSLANIARQRPMFMSEVIQAYETLHANLPPTLAKSQVSSVRKNLKLHLLSVLKHPASLEFQAQITTLLVDLGTPQAEIARNMPSSKDTRKRPRDDSDSTLKKMKLEPNLGEDDEDKDLEPGPSGTSKASAQISGQSDTDITAEFLQPLLTPDNVANLVLISMVYLPEAMPASFQAIYTPVESAGTEAQIKHLARLMATQMTAAGLGPGVEQTKQCKEEPKEEKVVKTESVLIKRRL Predict reactive species
Full Name	symplekin
Calculated Molecular Weight	1274 aa, 141 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	BC006567
Gene Symbol	Symplekin
Gene ID (NCBI)	8189
RRID	AB_2200281
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q92797
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for Symplekin antibody 11519-1-AP	Download protocol
IHC protocol for Symplekin antibody 11519-1-AP	Download protocol
IP protocol for Symplekin antibody 11519-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB,IF	Front Cell Dev Biol SYMPK Is Required for Meiosis and Involved in Alternative Splicing in Male Germ Cells Authors - Rui Wu KO Validated View Article