Sestrin2 Antibody
Rabbit Polyclonal
Catalog number: 10795-1-AP
Ask a question about this product click here
Loading price...
Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | IF, IHC, IP, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse |
| Positive WB detected in | HEK-293 cells, HeLa cells, human brain tissue, human liver tissue, K-562 cells |
| Positive IHC detected in | human colon cancer |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:50-1:200 IF: 1:20-1:50 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-SESN2 (10795-1-AP for IP and Detestion) with K562 cell.
HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP(Sestrin2 Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human colon cancer using 10795-1-AP(Sestrin2 Antibody) at Dilution 1:100 (under 25x lens)
Immunofluorescent analysis of Hela cells, using SESN2 antibody 10795-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | Sestrin2 fusion protein ag1247 | Full name | sestrin 2 |
|
Calculated molecular weight |
54kd |
Observed molecular
weight |
54-60kd |
|
GenBank accession number |
BC013304 | Gene ID (NCBI) | 83667 |
| Gene symbol | SESN2 | Synonyms | DKFZp761M0212; DKFZp761M02121; HI95; SES2; SEST2 |
Background
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids). This antibody is against Pan- Sestrins. |
