Sestrin2 AntibodyRabbit Polyclonal
|Tested applications||ELISA, WB, IHC, IF, IP|
|Cited applications||IF, IHC, IP, WB|
|Species specificity||human, mouse, rat; other species not tested.|
|Cited species||human, mouse|
|Positive WB detected in||HEK-293 cells, HeLa cells, human brain tissue, human liver tissue, K-562 cells|
|Positive IHC detected in||human colon cancer|
|Positive IF detected in||Hela cells|
|Recommended dilution||WB: 1:500-1:5000|
IP = Immunoprecipitation
- IP result of anti-SESN2 (10795-1-AP for IP and Detestion) with K562 cell.
- HEK-293 cells were subjected to SDS PAGE followed by western blot with 10795-1-AP(Sestrin2 Antibody) at dilution of 1:1000
- Immunohistochemistry of paraffin-embedded human colon cancer using 10795-1-AP(Sestrin2 Antibody) at Dilution 1:100 (under 25x lens)
- Immunofluorescent analysis of Hela cells, using SESN2 antibody 10795-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
|Source||Rabbit||Purification method||Antigen affinity purification|
|Isotype||IgG||Storage||PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC.|
|Immunogen||Sestrin2 fusion protein ag1247||Full name||sestrin 2|
|BC013304||Gene ID (NCBI)||83667|
|Gene symbol||SESN2||Synonyms||DKFZp761M0212; DKFZp761M02121; HI95; SES2; SEST2|
Sestrins, including sestrin-1 (PA26), sestrin-2 (Hi95), and sestrin-3, are 48 to 65 kDa cystein sulfinyl reductases and they modulate peroxide signaling and antioxidant defense. These proteins selectively reduce or repair hyperoxidized forms of typical 2-Cys peroxiredoxins within eukaryotes. Expression of these proteins is regulated by p53, a tumor suppressor protein. Sestrin 2 is implicated in the regulation of cell growth and survival, in cellular responses to different stress conditions, and also acts as a positive regulator of autophagy. Sestrin 2 is approximately a 57.6kDa protein (480 amino acids). This antibody is against Pan- Sestrins.
|ag1247||Sestrin2 fusion protein gst tag|
|ag15669||SESN2 fusion protein gst tag|
|ag15873||SESN2 fusion protein 6*his tag|