Tested Applications
| Positive WB detected in | HeLa cells, NIH/3T3 cells, human brain tissue, Jurkat cells |
| Positive IHC detected in | human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:9000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10839-1-AP targets TADA3L in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1276 Product name: Recombinant human TADA3L protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-369 aa of BC009240 Sequence: MSELKDCPLQFHDFKSVDHLKVCPRYTAVLARSEDDGIGIEELDTLQLELETLLSSASRRLRVLEAETQILTDWQDKKGDRRFLKLGRDHELGAPPKHGKPKKQKLEGKAGHGPGPGPGRPKSKNLQPKIQEYEFTDDPIDVPRIPKNDAPNRFWASVEPYCADITSEEVRTLEELLKPPEDEAEHYKIPPLGKHYSQRWAQEDLLEEQKDGARAAAVADKKKGLMGPLTELDTKDVDALLKKSEAQHEQPEDGCPFGALTQRLLQALVEENIISPMEDSPIPDMSGKESGADGASTSPRNQNKPFSVPHTKSLESRIKEELIAQGLLESEDRPAEDSEDEVLAELRKRQAELKALSAHNRTKKHDLLR Predict reactive species |
| Full Name | transcriptional adaptor 3 (NGG1 homolog, yeast)-like |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | BC009240 |
| Gene Symbol | TADA3L |
| Gene ID (NCBI) | 10474 |
| RRID | AB_513667 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O75528 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TADA3 is a subunit of 2 histone acetyltransferase complexes: the ADA2A (TADA2A)-containing (ATAC) complex and the SPT3 (SUPT3H)-TAF9-GCN5 (KAT2A)/PCAF (KAT2B) acetylase (STAGA) complex. The ATAC complex is a complex with histone acetyltransferase activity on histones H3 and H4, and the PCAF complex is capable of efficiently acetylating histones in a nucleosomal context. TADA3 is also known as a coactivator for p53/TP53-dependent transcriptional activation.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TADA3L antibody 10839-1-AP | Download protocol |
| WB protocol for TADA3L antibody 10839-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
