- Featured Product
- KD/KO Validated
TAF12 Polyclonal antibody
TAF12 Polyclonal Antibody for IP, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
WB, IP, IHC, ChIP, ELISA
Conjugate
Unconjugated
Cat no : 12353-1-AP
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | Y79 cells |
| Positive IP detected in | Y79 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
| IHC | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
12353-1-AP targets TAF12 in WB, IP, IHC, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | TAF12 fusion protein Ag3017 |
| Full Name | TAF12 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 20kDa |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC011986 |
| Gene Symbol | TAF12 |
| Gene ID (NCBI) | 6883 |
| RRID | AB_2271582 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TAF12, also named TAF15 or TAF2J, is a TBP-associated factor that is contained in Pol I- and Pol II-specific TBP-TAF complexes, it is also a component of the transcription factor IID (TFIID) complex, which is essential for mediating regulation of RNA polymerase transcription. TAF12 plays a key role in regulating eukaryotic gene expression by directly binding promoters and enhancer-bound transactivator proteins. Likewise, TAF12 recruits Gadd45a and the nucleotide excision repair complex to the promoter of rRNA genes leading to active DNA demethylation. Observed MW of TAF12 is from 14-17 kDa and 17-21 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TAF12 antibody 12353-1-AP | Download protocol |
| IP protocol for TAF12 antibody 12353-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Cancer Cell A TFIID-SAGA Perturbation that Targets MYB and Suppresses Acute Myeloid Leukemia.
| ||
Cell Metab Contributions of the measles virus nucleocapsid gene and the SQSTM1/p62(P392L) mutation to Paget's disease. | ||
J Bone Miner Res Role of ATF7-TAF12 interactions in the vitamin D response hypersensitivity of osteoclast precursors in Paget's disease. | ||
J Bone Miner Res TBK1 mediates critical effects of measles virus nucleocapsid protein (MVNP) on pagetic osteoclast formation. | ||
J Bone Miner Res Increased IL-6 expression in osteoclasts is necessary but not sufficient for the development of Paget's disease of bone. | ||
Mol Cell Biol The general transcription factor TAF7 is essential for embryonic development but not essential for the survival or differentiation of mature T cells. |
