Tested Applications
| Positive WB detected in | Jurkat cells, HEK-293 cells, HeLa cells, mouse brain tissue, SH-SY5Y cells, A549 cells |
| Positive IHC detected in | human colon cancer tissue, human cervical cancer tissue, human kidney tissue, human malignant melanoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:600-1:2400 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
25521-1-AP targets TAF15 in WB, IHC, IF, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa |
| Calculated Molecular Weight | 62 kDa |
| Observed Molecular Weight | 65-70 kDa |
| GenBank Accession Number | NM_003487 |
| Gene Symbol | TAF15 |
| Gene ID (NCBI) | 8148 |
| RRID | AB_2880116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92804 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TAF15 antibody 25521-1-AP | Download protocol |
| WB protocol for TAF15 antibody 25521-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-TAF15 antibody (Cat# 25521-1-AP) has 5 total citations published in Cancer Letters, International Journal of Biological Sciences, Journal of Biological Chemistry, Molecular Genetics and Genomics, and F1000Research. Research fields span cancer biology (lung adenocarcinoma vascular invasion, melanoma proliferation), erythroid gene regulation, epigenetics (H3K27 acetylation in cell migration), and antibody validation for WB, IP, and IF applications. All citations involve human samples.
| Species | Application | Title |
|---|---|---|
Cancer Lett A tumor-derived mitochondrial tsRNA drives vascular invasion of lung adenocarcinoma by promoting ribosomal assembly in endothelial cells. | ||
Int J Biol Sci The SOX10-ACAT2-Cholesterol Synthesis Axis Is Required for Melanoma Proliferation. | ||
J Biol Chem TFII-I/GTF2I regulates globin gene expression and stress response in erythroid cells | ||
Mol Genet Genomics UCMSC-derived exosomal circDLGAP4 enhances HFF-1 cell proliferation and migration by promoting ZNF217 promoter H3K27 acetylation.
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F1000Res A guide to selecting high-performing antibodies for TAF15 (UniProt ID: Q92804) for use in western blot, immunoprecipitation, and immunofluorescence
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Reviews
What customers say
One user rated this antibody 3 out of 5 stars. The reviewer used it for Western Blot on HEK293T cells at a 1:1000 dilution with 5% blocking milk and reported that multiple bands appeared on the blot, suggesting some lack of specificity under their conditions. Overall, the feedback is mixed, with the main concern being band specificity rather than complete failure of detection.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Manohar (Verified Customer) (05-13-2020) | There are multiple bands appearing on western blot. When used with 5% blocking milk and 1:1000 antibody dilution
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