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WB analysis of human liver using 10158-2-AP
human liver tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of human brain using 10158-2-AP
human brain tissue were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10158-2-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 10158-2-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 10158-2-AP (TAOK3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
IP experiment of HepG2 using 10158-2-AP
IP result of anti-TAOK3 (IP:10158-2-AP, 4ug; Detection:10158-2-AP 1:700) with HepG2 cells lysate 3600ug.
IHC staining of human liver using 10158-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 10x lens).
IHC staining of human liver using 10158-2-AP
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 10158-2-AP (TAOK3 Antibody) at dilution of 1:200 (under 40x lens).
IHC staining of human liver using 10158-2-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 10158-2-AP
Immunohistochemical analysis of paraffin-embedded human liver using 10158-2-AP (TAOK3 antibody) at dilution of 1:50 (under 40x lens).

Featured Product
KD/KO Validated

TAOK3 Polyclonal antibody

Name: TAOK3 Polyclonal antibody
Brand: Proteintech
SKU: 10158-2-AP

TAOK3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10158-2-AP

Publications(3)

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Synonyms

DPK, hKFC A, JIK, JNK/SAPK inhibitory kinase, Jun kinase inhibitory kinase, KDS, Kinase from chicken homolog A, MAP3K18, TAO kinase 3, TAOK3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Publications (3)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	human liver tissue, HeLa cells, HepG2 cells, human brain tissue
Positive IP detected in	HepG2 cells
Positive IHC detected in	human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:100-1:400
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 2 publications below
IP	See 1 publications below

Product Information

10158-2-AP targets TAOK3 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0211 Product name: Recombinant human TAOK3 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-240 aa of BC002756 Sequence: MRKGVLKDPEIADLFYKDDPEELFIGLHEIGHGSFGAVYFATNAHTSEVVAIKKMSYSGKQTHEKWQDILKEVKFLRQLKHPNTIEYKGCYLKEHTAWLVMEYCLGSASDLLEVHKKPLQEVEIAAITHGALHGLAYLHSHALIHRDIKAGNILLTEPGQVKLADFGSASMASPANSFVGTPYWMAPEVILAMDEGQYDGKVDIWSLGITCIELAERKPPLFNMNAMSALYHIAQNDSPT Predict reactive species
Full Name	TAO kinase 3
Calculated Molecular Weight	105 kDa
Observed Molecular Weight	100-105 kDa
GenBank Accession Number	BC002756
Gene Symbol	TAOK3
Gene ID (NCBI)	51347
RRID	AB_2255688
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9H2K8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TAOK3(Serine/threonine-protein kinase TAO3) is also named as DPK, JIK, KDS, MAP3K18 and belongs to the STE Ser/Thr protein kinase family. It acts as an activator of the p38/MAPK14 stress-activated MAPK cascade. In response to DNA damage, it is involved in the G2/M transition DNA damage checkpoint by activating the p38/MAPK14 stress-activated MAPK cascade, probably by mediating phosphorylation of upstream MAP2K3 and MAP2K6 kinases. And this protein can be autophosphorylated(PMID:20949042).

Publications

Species	Application	Title
human	WB	Mol Carcinog Targeting TAOK1 with resveratrol inhibits esophageal squamous cell carcinoma growth in vitro and in vivo Authors - Mengqiu Song View Article
human	WB	Cell Commun Signal Kinase shRNA screening reveals that TAOK3 enhances microtubule-targeted drug resistance of breast cancer cells via the NF-κB signaling pathway. Authors - Tsung-Ching Lai KD Validated View Article
mouse	IP	Sci Signal The induction of SHP-1 degradation by TAOK3 ensures the responsiveness of T cells to TCR stimulation Authors - Alexandre Poirier View Article