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  • Featured Product
  • KD/KO Validated

TASP1 Polyclonal antibody

TASP1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 16739-1-AP

Synonyms

C20orf13, TASP1, Taspase 1, Threonine aspartase 1

Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using TASP1 Polyclonal antibody (16739-1-AP)

    WB analysis of HEK-293 using 16739-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TASP1 Polyclonal antibody (16739-1-AP)

    WB analysis of HeLa using 16739-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TASP1 Polyclonal antibody (16739-1-AP)

    WB analysis of HeLa using 16739-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 16739-1-AP (TASP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human ovary tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human ovary using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human ovary tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human ovary using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human kidney using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human kidney tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human kidney using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human kidney using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human testis using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human testis tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human testis using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human testis using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human liver using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human liver using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human lung using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human lung tissue using TASP1 Polyclonal antibody (16739-1-AP)

    IHC staining of human lung using 16739-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung using 16739-1-AP (TASP1 antibody) at dilution of 1:100 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using TASP1 Polyclonal antibody (16739-1-AP)

    IF Staining of HeLa using 16739-1-AP

    Immunofluorescent analysis of HeLa cells using 16739-1-AP (TASP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHEK-293 cells, HeLa cells
Positive IHC detected inhuman ovary tissue, human kidney tissue, human liver tissue, human lung tissue, human testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

16739-1-AP targets TASP1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TASP1 fusion protein Ag9918
Full Name taspase, threonine aspartase, 1
Calculated Molecular Weight 420 aa, 44 kDa
Observed Molecular Weight 50 kDa
GenBank Accession NumberBC025266
Gene Symbol TASP1
Gene ID (NCBI) 55617
RRIDAB_2201218
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TASP1 antibody 16739-1-APDownload protocol
IHC protocol for TASP1 antibody 16739-1-APDownload protocol
IF protocol for TASP1 antibody 16739-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Hum Mol Genet

Suleiman-El-Hattab syndrome: a histone modification disorder caused by TASP1 deficiency.

Authors - Korbinian M Riedhammer
  • KD Validated
View Article
humanWB,IHC

Cancer Manag Res

Overexpression of Taspase 1 Predicts Poor Prognosis in Patients with Hepatocellular Carcinoma.

Authors - Jie Jiang
View Article