TBC1D17 Polyclonal antibody

TBC1D17 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 20482-1-AP

Synonyms

TBC1 domain family member 17, TBC1 domain family, member 17, TBC1D17



Tested Applications

Positive WB detected inmouse brain tissue, mouse thymus tissue, Jurkat cells, rat brain tissue
Positive IP detected inJurkat cells
Positive IHC detected inhuman breast cancer tissue, human liver cancer tissue, human heart tissue, mouse testis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

20482-1-AP targets TBC1D17 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBC1D17 fusion protein Ag14358
Full Name TBC1 domain family, member 17
Calculated Molecular Weight 648 aa, 73 kDa
Observed Molecular Weight 73 kDa
GenBank Accession NumberBC003516
Gene Symbol TBC1D17
Gene ID (NCBI) 79735
RRIDAB_11232420
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TBC1D17 antibody 20482-1-APDownload protocol
IHC protocol for TBC1D17 antibody 20482-1-APDownload protocol
IF protocol for TBC1D17 antibody 20482-1-APDownload protocol
IP protocol for TBC1D17 antibody 20482-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Chun (Verified Customer) (06-19-2022)

This antibody worked well.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1,000
  • Cell Tissue Type: HeLa cells