TBCC Polyclonal antibody

TBCC Polyclonal Antibody for FC, IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 15917-1-AP

Synonyms

CFC, TBCC, tubulin folding cofactor C, Tubulin specific chaperone C



Tested Applications

Positive WB detected inHepG2 cells, human brain tissue, HeLa cells, K-562 cells, MCF-7 cells, rat liver tissue
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunofluorescence (IF)IF : 1:10-1:100
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

15917-1-AP targets TBCC in WB, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBCC fusion protein Ag8719
Full Name tubulin folding cofactor C
Calculated Molecular Weight 346 aa, 39 kDa
Observed Molecular Weight 39 kDa
GenBank Accession NumberBC020170
Gene Symbol TBCC
Gene ID (NCBI) 6903
RRIDAB_2199538
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBCC, also named CFC. Expressed in the retina. Expressed in the rod and cone photoreceptors, extending from the inner segments (IS), through the outer nuclear layer (ONL) and into the synapses in the outer plexiform layer (OPL). Strongly expressed to the photoreceptor connecting cilium at the tips of the IS (at protein level). Cofactor C was cytoplasmic in distribution (PMID: 12417528).

Protocols

Product Specific Protocols
WB protocol for TBCC antibody 15917-1-APDownload protocol
IF protocol for TBCC antibody 15917-1-APDownload protocol
FC protocol for TBCC antibody 15917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Histochem Cell Biol

Spatio-temporal distribution of tubulin-binding cofactors and posttranslational modifications of tubulin in the cochlea of mice.

Authors - Lukas Juergens