TBP Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB |
| Cited applications | WB |
| Species specificity | human, mouse; other species not tested. |
| Cited species | human |
| Positive WB detected in | COLO 320 cells |
| Recommended dilution | WB: 1:500-1:5000 |
IP = Immunoprecipitation
COLO 320 cells were subjected to SDS PAGE followed by western blot with 22006-1-AP(TBP Antibody) at dilution of 1:1500
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | TBP fusion protein ag12383 | Full name | TATA box binding protein |
|
Calculated molecular weight |
338aa,38kd |
Observed molecular
weight |
43kd |
|
GenBank accession number |
BC110341 | Gene ID (NCBI) | 6908 |
| Gene symbol | TBP | Synonyms | GTF2D; GTF2D1; MGC117320; MGC126054; MGC126055; SCA17; TFIID |
The TATA binding protein (TBP) is a transcription factor that binds specifically to a DNA sequence TATA box. This DNA sequence is found about 25-30 base pairs upstream of the transcription start site in some eukaryotic gene promoters. TBP, along with a variety of TBP-associated factors, make up the TFIID, a general transcription factor that in turn makes up part of the RNA polymerase II preinitiation complex. As one of the few proteins in the preinitation complex that binds DNA in a sequence-specific manner, it helps position RNA polymerase II over the transcription start site of the gene. However, it is estimated that only 10-20% of human promoters have TATA boxes. Therefore, TBP is probably not the only protein involved in positioning RNA polymerase II. This protein is not suitable for samples where the nuclear envelope has been removed. |
