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  • Featured Product
  • KD/KO Validated

TBXA2R Polyclonal antibody

TBXA2R Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (1)

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 27159-1-AP

Synonyms

Prostanoid TP receptor, TBXA2R, thromboxane A2 receptor, TP, TXA2 R

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using TBXA2R Polyclonal antibody (27159-1-AP)

    WB analysis using 27159-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of human placenta tissue using TBXA2R Polyclonal antibody (27159-1-AP)

    WB analysis of human placenta using 27159-1-AP

    human placenta tissue were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of DU 145 cells using TBXA2R Polyclonal antibody (27159-1-AP)

    WB analysis of DU 145 using 27159-1-AP

    DU 145 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using TBXA2R Polyclonal antibody (27159-1-AP)

    WB analysis of MCF-7 using 27159-1-AP

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of PC-3 cells using TBXA2R Polyclonal antibody (27159-1-AP)

    WB analysis of PC-3 using 27159-1-AP

    PC-3 cells were subjected to SDS PAGE followed by western blot with 27159-1-AP (TBXA2R Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TBXA2R Polyclonal antibody (27159-1-AP)

    IHC staining of human prostate cancer using 27159-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using TBXA2R Polyclonal antibody (27159-1-AP)

    IHC staining of human prostate cancer using 27159-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBXA2R Polyclonal antibody (27159-1-AP)

    IHC staining of human breast cancer using 27159-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using TBXA2R Polyclonal antibody (27159-1-AP)

    IHC staining of human breast cancer using 27159-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 27159-1-AP (TBXA2R Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Tested Applications

Positive WB detected inHT-29 cells, DU 145 cells, human placenta tissue, MCF-7 cells, PC-3 cells, Hela cells
Positive IHC detected inhuman prostate cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:16000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 27159-1-AP is TBXA2R Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivitymouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TBXA2R fusion protein Ag25670
Full Name thromboxane A2 receptor
Calculated Molecular Weight343aa,37 kDa; 1043aa,113 kDa
Observed Molecular Weight 50-60 kDa
GenBank Accession NumberBC074750
Gene Symbol TBXA2R
Gene ID (NCBI) 6915
RRIDAB_2880782
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TBXA2R, also named as thromboxane A2 receptor, TXA2-R and Prostanoid TP receptor, belongs to G-protein coupled receptor 1 family. Thromboxane A2 receptor is a key molecule in hemostasis as its abnormality leads to bleeding disorders. The TXA2-R receptor is linked via a guanine nucleotide-binding protein (G protein) to phospholipase C (PLC), which hydrolyses phosphoinositides to the two potent stimulatory second messengers inositol 1,4,5-triphosphate (IP3) and diacylglycerol. IP3 causes increases in cytoplasmic free calcium and diacylglycerol causes activation of protein kinase C. In the kidney, the binding of TXA2-R to glomerular TP receptors causes intense vasoconstriction. The two isoforms expressed in cultured cells show similar ligand binding characteristics and phospholipase C (PLC) activation but oppositely regulate adenylyl cyclase activity (PMID: 8613548).

Protocols

Product Specific Protocols
WB protocol for TBXA2R antibody 27159-1-APDownload protocol
IHC protocol for TBXA2R antibody 27159-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Am J Respir Crit Care Med

Thromboxane-Prostanoid Receptor Signaling Drives Persistent Fibroblast Activation in Pulmonary Fibrosis

Authors - Toshio Suzuki
  • KO Validated
View Article
ratWB, IHC

Front Endocrinol (Lausanne)

The Arachidonic Acid Metabolism Mechanism Based on UPLC-MS/MS Metabolomics in Recurrent Spontaneous Abortion Rats.

Authors - Meihe Li
View Article