Tested Applications
| Positive WB detected in | HeLa cells, Neuro-2a cells, Jurkat cells |
| Positive IHC detected in | human colon cancer tissue, human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MCF-7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11003-1-AP targets TCOF1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1447 Product name: Recombinant human TCOF1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-350 aa of BC014559 Sequence: MAEARKRRELLPLIYHHLLRAGYVRAAREVKEQSGQKCFLAQPVTLLDIYTHWQQTSELGRKRKAEEDAALQAKKTRVSDPISTSESSEEEEEAEAETAKATPRLASTNSSVLGADLPSSMKEKAKAETEKAGKTGNSMPHPATGKTVANLLSGKSPRKSAEPSANTTLVSETEEEGSVPAFGAAAKPGMVSAGQADSSSEDTSSSSDETDVEGKPSVKPAQVKASSVSTKESPARKAAPAPGKVGDVTPQVKGGALPPAKRAKKPEEESESSEEGSESEEEAPAGTRSQVKASEKILQVRAASAPAKGTPGKGATPAPPGKAGAVASQTKAGKPEEDSESSSEESSDSE Predict reactive species |
| Full Name | Treacher Collins-Franceschetti syndrome 1 |
| Calculated Molecular Weight | 97 kDa, 144 kDa, 152 kDa |
| Observed Molecular Weight | 220 kDa |
| GenBank Accession Number | BC014559 |
| Gene Symbol | TCOF1 |
| Gene ID (NCBI) | 6949 |
| RRID | AB_2202516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q13428 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TCOF1 also known as treacle, is a nucleolar protein with a LIS1 homology domain. TCOF1 is involved in ribosomal DNA gene transcription by interacting with upstream binding factor (UBF). Mutations in TCOF1 have been associated with Treacher Collins syndrome, a disorder which includes abnormal craniofacial development(PMID: 31218872)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| IHC protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| WB protocol for TCOF1 antibody 11003-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Antibody 11003-1-AP (anti-TCOF1/Treacle) has 13 total citations published in Nature, Cell, Nature Communications, PNAS, Cell Reports, International Journal of Molecular Sciences, Cancer Science, Medical Oncology, Viruses, PLoS ONE, Traffic, and eLife. Research fields include cell fate determination, biomolecular condensates and phase separation, viral nucleolar biology, DNA damage response, ribosomal biogenesis, hepatocellular carcinoma, colorectal cancer progression, and CRISPR/Cas9 gene editing.
| Species | Application | Title |
|---|---|---|
Nat Commun Conformational dynamics, RNA binding, and phase separation regulate the multifunctionality of rabies virus P protein. | ||
Nat Commun Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response. | ||
Proc Natl Acad Sci U S A Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.
| ||
Cell Rep An evolutionarily nascent architecture underlying the formation and emergence of biomolecular condensates |
