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  • Featured Product
  • KD/KO Validated

TCOF1 Polyclonal antibody

TCOF1 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 11003-1-AP

Synonyms

MFD1, TCOF1, treacle, Treacle protein

Validation Data Gallery

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  • Western Blot (WB) analysis of Jurkat cells using TCOF1 Polyclonal antibody (11003-1-AP)

    WB analysis of Jurkat using 11003-1-AP

    WB result of TCOF1 antibody (11003-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-TCOF1 transfected Jurkat cells.

  • Western Blot (WB) analysis of HeLa cells using TCOF1 Polyclonal antibody (11003-1-AP)

    WB analysis of HeLa using 11003-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using TCOF1 Polyclonal antibody (11003-1-AP)

    WB analysis of HeLa using 11003-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Neuro-2a cells using TCOF1 Polyclonal antibody (11003-1-AP)

    WB analysis of Neuro-2a using 11003-1-AP

    Neuro-2a cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using TCOF1 Polyclonal antibody (11003-1-AP)

    WB analysis of Jurkat using 11003-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 11003-1-AP (TCOF1 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TCOF1 Polyclonal antibody (11003-1-AP)

    IHC staining of human colon cancer using 11003-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using TCOF1 Polyclonal antibody (11003-1-AP)

    IHC staining of human colon cancer using 11003-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using TCOF1 Polyclonal antibody (11003-1-AP)

    IHC staining of human lymphoma using 11003-1-AP

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 11003-1-AP (TCOF1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TCOF1 Polyclonal antibody (11003-1-AP)

    IF Staining of MCF-7 using 11003-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using TCOF1 antibody (11003-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using TCOF1 Polyclonal antibody (11003-1-AP)

    IF Staining of MCF-7 using 11003-1-AP

    Immunofluorescent analysis of MCF-7 cells, using TCOF1 antibody 11003-1-AP at 1:50 dilution and FITC-labeled donkey anti-rabbit IgG(green). Blue pseudocolor = DAPI (fluorescent DNA dye).

Tested Applications

Positive WB detected inHeLa cells, Neuro-2a cells, Jurkat cells
Positive IHC detected inhuman colon cancer tissue, human lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

11003-1-AP targets TCOF1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TCOF1 fusion protein Ag1447
Full Name Treacher Collins-Franceschetti syndrome 1
Calculated Molecular Weight 97 kDa, 144 kDa, 152 kDa
Observed Molecular Weight 220 kDa
GenBank Accession NumberBC014559
Gene Symbol TCOF1
Gene ID (NCBI) 6949
RRIDAB_2202516
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for TCOF1 antibody 11003-1-APDownload protocol
IHC protocol for TCOF1 antibody 11003-1-APDownload protocol
IF protocol for TCOF1 antibody 11003-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Nature

Cell-fate determination by ubiquitin-dependent regulation of translation.

Authors - Achim Werner
View Article
human

Nat Commun

Viral regulation of host cell biology by hijacking of the nucleolar DNA-damage response.

Authors - Stephen M Rawlinson
View Article
humanWB, IF

Proc Natl Acad Sci U S A

Treacher Collins syndrome TCOF1 protein cooperates with NBS1 in the DNA damage response.

Authors - Alberto Ciccia
  • KD Validated
View Article
humanWB

Elife

Multisite dependency of an E3 ligase controls monoubiquitylation-dependent cell fate decisions.

Authors - Achim Werner
View Article
humanWB, IF

Int J Mol Sci

TRPV1 Activation Promotes β-arrestin2 Interaction with the Ribosomal Biogenesis Machinery in the Nucleolus:Implications for p53 Regulation and Neurite Outgrowth.

Authors - Ahmed Hassan
View Article
humanWB,IF

Cancer Sci

TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC.

Authors - Chaoshen Wu
View Article