TFEB Antibody
Rabbit Polyclonal
Catalog number: 13372-1-AP
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Applications
| Tested applications | ELISA, WB, IHC, IF, IP |
| Cited applications | |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | |
| Positive WB detected in | Jurkat cells, HeLa cells, human placenta tissue, NIH/3T3 cells |
| Positive IHC detected in | human renal cell carcinoma |
| Positive IF detected in | Hela cells |
| Recommended dilution | WB: 1:500-1:5000 IHC: 1:20-1:200 IF: 1:20-1:100 |
Application key: WB = Western blotting, IHC = Immunohistochemistry, IF = Immunofluorescence,
IP = Immunoprecipitation
IP = Immunoprecipitation
Images
IP result of anti-TFEB (13372-1-AP for IP and Detection) with NIH3T3 cell lysate.
Jurkat cells were subjected to SDS PAGE followed by western blot with 13372-1-AP(TFEB Antibody) at dilution of 1:1000
Immunohistochemistry of paraffin-embedded human renal cell carcinoma using 13372-1-AP(TFEB Antibody) at Dilution 1:50 (under 10x lens)
Immunohistochemistry of paraffin-embedded human renal cell carcinoma using 13372-1-AP(TFEB Antibody) at Dilution 1:50 (under 40x lens)
Immunofluorescent analysis of Hela cells, using TFEB antibody 13372-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).
Product Information
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
Immunogen Information
| Immunogen | TFEB fusion protein ag4194 | Full name | transcription factor EB |
|
Calculated molecular weight |
476aa,53kd |
Observed molecular
weight |
65-70kd |
|
GenBank accession number |
BC032448 | Gene ID (NCBI) | 7942 |
| Gene symbol | TFEB | Synonyms | AlphaTFEB; TCFEB; bHLHe35 |
Background
Transcription factor EB(TFEB) is one of the transcription factors that contain a basic HLH-ZIP domain(for DNA binding ) and HLH and ZIP domains( for loigomerization). TFEB specifically recognizes and binds to E-box sequences, which required dimerization with itself or another MiT/TFE family member. It plays a role in T-cell-dependent antibody responses to activated CD4+ T-cells and thymus-dependent humoral immunity once associated with TFE3. It also activates lysosomal gene by binding the CLEAR-box sequence in the regulatory region. It involves in the signal transduction processes required for normal vascularization of the placenta. This is a rabbit polyclonal antibody raised against the full length of human TFEB |
