THAP1 Antibody
Rabbit Polyclonal
| Tested applications | ELISA, WB, IHC |
| Cited applications | IHC, WB |
| Species specificity | human, mouse, rat; other species not tested. |
| Cited species | human, mouse, rat |
| Positive WB detected in | HEK-293 cells, A375 cells, HeLa cells, HepG2 cells, human brain tissue, human heart tissue, mouse brain tissue, mouse heart tissue, SGC-7901 cells |
| Positive IHC detected in | human colon cancer |
| Recommended dilution | WB: 1:200-1:2000 IHC: 1:20-1:200 |
IP = Immunoprecipitation
HEK-293 cells were subjected to SDS PAGE followed by western blot with 12584-1-AP(THAP1 Antibody) at dilution of 1:800
Immunohistochemistry of paraffin-embedded human colon cancer using 12584-1-AP(THAP1 Antibody) at Dilution 1:50 (under 10x lens)
| Source | Rabbit | Purification method | Antigen affinity purification |
| Isotype | IgG | Storage | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. Store at -20oC. |
| Immunogen | THAP1 fusion protein ag3285 | Full name | THAP domain containing, apoptosis associated protein 1 |
|
Calculated molecular weight |
213aa,25kd |
Observed molecular
weight |
30kd |
|
GenBank accession number |
BC021721 | Gene ID (NCBI) | 55145 |
| Gene symbol | THAP1 | Synonyms | FLJ10477; MGC33014 |
THAP1 belongs to the THAP1 family. It is a DNA-binding transcription regulator that regulates endothelial cell proliferation and G1/S cell-cycle progression. THAP1 may also have pro-apoptopic activity by potentiating both serum-withdrawal and TNF-induced apoptosis. Mutations in THAP1 have been associated with dystonia 6. THAP1 encodes a transcription factor with mostly unknown targets. It regulates the expression of DYT1 (TOR1A), another dystonia-causing gene. After characterization of the TOR1A promoter, THAP1 binds to the core promoter of TOR1A. Wild type THAP1 represses the expression of TOR1A, whereas dystonia 6-associated mutant THAP1 results in decreased repression of TOR1A. Catalog#12584-1-AP is a rabbit polyclonal antibody raised against full-length of huamn THAP1. |
