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THOC2 Polyclonal antibody

THOC2 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 55178-1-AP

Publications(7)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, IP, CoIP, RIP, ELISA

Synonyms

CXorf3, hTREX120, THO complex 2, THO complex subunit 2, Tho2

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

-/ -

Freight/Packing: -

Quantity

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Filter: AllWBIPIF/ICC

WB analysis of HeLa using 55178-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 55178-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of Jurkat using 55178-1-AP
Jurkat cells were subjected to SDS PAGE followed by western blot with 55178-1-AP (THOC2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IP experiment of HeLa using 55178-1-AP
IP result of anti-THOC2 (IP:55178-1-AP, 4ug; Detection:55178-1-AP 1:600) with HeLa cells lysate 1600 ug.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 55178-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using THOC2 antibody (55178-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of HeLa using 55178-1-AP
Immunofluorescent analysis of HeLa cells using 55178-1-AP (THOC2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Overview

Tested Applications
Product Information
Publications (7)
Protocols
Reviews (1)

Print datasheet

MSDS SDS

Tested Applications

Positive WB detected in	HEK-293T cells, HeLa cells, Jurkat cells
Positive IP detected in	HeLa cells
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

55178-1-AP targets THOC2 in WB, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	THO complex 2
Calculated Molecular Weight	183 kDa
Observed Molecular Weight	150 kDa
GenBank Accession Number	NM_020449
Gene Symbol	THOC2
Gene ID (NCBI)	57187
RRID	AB_2881283
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q8NI27
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

THOC2 also termed as THO complex 2 or CXorf3 is 1593 amino acid protein, which belongs to the THOC2 family. THOC2 is a component of heteromultimeric THO/TREX (transcription/export) complex, which is a multi-protein structure consisting of THOC1, THOC2, THOC3, BAT1 and ALY. The TREX complex associates with spliced mRNA and not with unspliced pre-mRNA. It is recruited to spliced mRNAs by a transcription-independent mechanism.

Protocols

Product Specific Protocols
IF protocol for THOC2 antibody 55178-1-AP	Download protocol
IP protocol for THOC2 antibody 55178-1-AP	Download protocol
WB protocol for THOC2 antibody 55178-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

Species	Application	Title
human	WB	Hum Mol Genet Clinical and functional characterization of recurrent missense variants implicated in THOC6-related intellectual disability. Authors - Mattioli Francesca View Article
human	WB	Viruses Murine Leukemia Virus Uses TREX Components for Efficient Nuclear Export of Unspliced Viral Transcripts. Authors - Toshie Sakuma KD Validated View Article
human	WB	Mol Cell YTHDC1 cooperates with the THO complex to prevent RNA-damage-induced DNA breaks Authors - Ning Tsao View Article
human	WB	Int J Biol Macromol Targeting mRNA export complex macromolecules THO subunits (Thoc2 and Thoc5) for somatic cell reprograming Authors - Abdur Rehman View Article
human	WB,CoIP	PLoS Biol CUL5 E3 ubiquitin ligase regulates the evasion of bladder cancer cells to CD8+ T cell-mediated killing by inhibiting autophagy. Authors - Xincheng Gao View Article
human	WB,RIP	Int J Biol Sci Targeting THOC2-Mediated mRNA Export Induces PARP Inhibitor Vulnerability in DNA Repair-Competent Hepatocellular Carcinoma. Authors - Xinli Li View Article