THTPA Polyclonal antibody

THTPA Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15486-1-AP

Synonyms

thiamine triphosphatase, THTP, THTPA, THTPASE



Tested Applications

Positive WB detected inK-562 cells, A431 cells, HeLa cells, human brain tissue, mouse testis tissue, mouse uterus tissue, PC-3 cells
Positive IP detected inK-562 cells
Positive IHC detected inhuman stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

15486-1-AP targets THTPA in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THTPA fusion protein Ag7834
Full Name thiamine triphosphatase
Calculated Molecular Weight 26 kDa
Observed Molecular Weight 26-35 kDa
GenBank Accession NumberBC002984
Gene Symbol THTPA
Gene ID (NCBI) 79178
RRIDAB_2203183
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THTPA antibody 15486-1-APDownload protocol
IHC protocol for THTPA antibody 15486-1-APDownload protocol
IF protocol for THTPA antibody 15486-1-APDownload protocol
IP protocol for THTPA antibody 15486-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

PLoS One

Metabolic effects of acute thiamine depletion are reversed by rapamycin in breast and leukemia cells.

Authors - Shuqian Liu