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THUMPD1 Polyclonal antibody

THUMPD1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 14921-1-AP

Synonyms

THUMP domain containing 1, THUMPD1

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis using 14921-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis of K-562 using 14921-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using THUMPD1 Polyclonal antibody (14921-1-AP)

    WB analysis of MCF-7 using 14921-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 14921-1-AP (THUMPD1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THUMPD1 Polyclonal antibody (14921-1-AP)

    IHC staining of human breast cancer using 14921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using THUMPD1 Polyclonal antibody (14921-1-AP)

    IHC staining of human breast cancer using 14921-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 14921-1-AP (THUMPD1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using THUMPD1 Polyclonal antibody (14921-1-AP)

    IF Staining of MCF-7 using 14921-1-AP

    Immunofluorescent analysis of (10% Formaldehyde) fixed MCF-7 cells using 14921-1-AP (THUMPD1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inHeLa cells, K-562 cells, MCF-7 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14921-1-AP targets THUMPD1 in WB, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen THUMPD1 fusion protein Ag6739
Full Name THUMP domain containing 1
Calculated Molecular Weight 39 kDa
Observed Molecular Weight 49 kDa
GenBank Accession NumberBC000448
Gene Symbol THUMPD1
Gene ID (NCBI) 55623
RRIDAB_2271785
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for THUMPD1 antibody 14921-1-APDownload protocol
IHC protocol for THUMPD1 antibody 14921-1-APDownload protocol
IF protocol for THUMPD1 antibody 14921-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Biosci Rep

Pan-cancer analysis of N4-acetylcytidine adaptor THUMPD1 as a predictor for prognosis and immunotherapy.

Authors - Kuangxun Li
View Article
humanIHC

iScience

RNA modification-related genes illuminate prognostic signature and mechanism in esophageal squamous cell carcinoma

Authors - Hui Han
View Article