Tie1 Polyclonal antibody

Tie1 Polyclonal Antibody for WB, ELISA

Cat No. 19329-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF, ELISA

Synonyms

JTK14, TIE, TIE1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of K-562 using 19329-1-AP
K-562 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of NIH/3T3 using 19329-1-AP
NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (Tie1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of MCF-7 using 19329-1-AP
MCF7 cells were subjected to SDS PAGE followed by western blot with 19329-1-AP (TIE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

K-562 cells, MCF-7 cells, NIH/3T3 cells

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

19329-1-AP targets Tie1 in WB, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag4728 Product name: Recombinant human TIE1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 849-1138 aa of BC038239 Sequence: NFGQVIRAMIKKDGLKMNAAIKMLKEYASENDHRDFAGELEVLCKLGHHPNIINLLGACKNRGYLYIAIEYAPYGNLLDFLRKSRVLETDPAFAREHGTASTLSSRQLLRFASDAANGMQYLSEKQFIHRDLAARNVLVGENLASKIADFGLSRGEEVYVKKTMGRLPVRWMAIESLNYSVYTTKSDVWSFGVLLWEIVSLGGTPYCGMTCAELYEKLPQGYRMEQPRNCDDEVYELMRQCWRDRPYERPPFAQIALQLGRMLEARKAYVNMSLFENFTYAGIDATAEEA Predict reactive species
Full Name	tyrosine kinase with immunoglobulin-like and EGF-like domains 1
Calculated Molecular Weight	1138 aa, 125 kDa
Observed Molecular Weight	154 kDa
GenBank Accession Number	BC038239
Gene Symbol	TIE1
Gene ID (NCBI)	7075
RRID	AB_10863655
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P35590
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
WB protocol for Tie1 antibody 19329-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	IF	Nat Commun Engineered model of heart tissue repair for exploring fibrotic processes and therapeutic interventions Authors - Pengcheng Yang View Article
mouse		Nature Bone marrow niches orchestrate stem-cell hierarchy and immune tolerance Authors - Kazuhiro Furuhashi View Article