Tested Applications
| Positive WB detected in | HeLa cells, K-562 cells, MCF-7 cells, Neuro-2a cells, NIH/3T3 cells, U-251 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
11189-1-AP targets TIMM17A in WB, IHC, IF-P, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1665 Product name: Recombinant human TIMM17A protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-171 aa of BC015098 Sequence: MEEYAREPCPWRIVDDCGGAFTMGTIGGGIFQAIKGFRNSPVGVNHRLRGSLTAIKTRAPQLGGSFAVWGGLFSMIDCSMVQVRGKEDPWNSITSGALTGAILAARNGPVAMVGSAAMGGILLALIEGAGILLTRFASAQFPNGPQFAEDPSQLPSTQLPSSPFGDYRQYQ Predict reactive species |
| Full Name | translocase of inner mitochondrial membrane 17 homolog A (yeast) |
| Calculated Molecular Weight | 18 kDa |
| Observed Molecular Weight | 18 kDa |
| GenBank Accession Number | BC015098 |
| Gene Symbol | TIMM17A |
| Gene ID (NCBI) | 10440 |
| RRID | AB_2271661 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q99595 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for TIMM17A antibody 11189-1-AP | Download protocol |
| IHC protocol for TIMM17A antibody 11189-1-AP | Download protocol |
| WB protocol for TIMM17A antibody 11189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The TIMM17A Polyclonal antibody (Cat# 11189-1-AP) has 10 citations published in journals including Theranostics, PNAS, Cell Reports, Journal of Cell Biology, EMBO Reports, Journal of Molecular and Cellular Cardiology, Proteomics, Cancer Research Communications, International Journal of Molecular Medicine, and Journal of Pharmaceutical and Biomedical Analysis. Research fields span mitochondrial biology, mitophagy, protein import, ER proteostasis, bone tissue engineering, stroke, breast cancer, lung cancer, and forensic pathology.
| Species | Application | Title |
|---|---|---|
Theranostics Vine-inspired zinc-ion modified black phosphorus coating accelerates bone tissue infiltration of 3D printed scaffolds | ||
Proc Natl Acad Sci U S A Mitochondrial ROS triggers mitophagy through activating the DNA damage response signaling pathway. | ||
Int J Mol Med 'Governor vessel-unblocking and mind-regulating' acupuncture therapy ameliorates cognitive dysfunction in a rat model of middle cerebral artery occlusion. | ||
Cell Rep TIM23 facilitates PINK1 activation by safeguarding against OMA1-mediated degradation in damaged mitochondria | ||
J Cell Biol ROMO1 is a constituent of the human presequence translocase required for YME1L protease import. | ||
EMBO Rep UBXN1 maintains ER proteostasis and represses UPR activation by modulating translation |
Reviews
What customers say
One customer reviewed this product for Western Blot on heart tissue at a 1:1000 dilution (Lot 00099503), giving it a 4-star rating and commenting "good." The review was posted in December 2024 by Angela Zhu. Overall, the limited feedback suggests satisfactory performance for WB applications, though the brief comment provides minimal detail about specificity, band quality, or troubleshooting experience.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Angela (Verified Customer) (12-27-2024) | good
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