TIMMDC1 Polyclonal antibody

TIMMDC1 Polyclonal Antibody for WB, IF-P, ELISA

Cat No. 23622-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF-P, ELISA

Synonyms

C3orf1, Complex I assembly factor TIMMDC1, mitochondrial, Protein M5 14, Protein M5-14, TIMM domain containing-protein 1

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWBIF-P

WB analysis of A375 using 23622-1-AP
A375 cells were subjected to SDS PAGE followed by western blot with 23622-1-AP (TIMMDC1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
IF Staining of mouse brain using 23622-1-AP
Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse brain tissue using TIMMDC1 antibody (23622-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in	A375 cells
Positive IF-P detected in	mouse brain tissue

Application	Dilution
Western Blot (WB)	WB : 1:500-1:2000
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

23622-1-AP targets TIMMDC1 in WB, IF-P, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag20017 Product name: Recombinant human TIMMDC1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-285 aa of BC012341 Sequence: MEVPPPAPRSFLCRALCLFPRVFAAEAVTADSEVLEERQKRLPYVPEPYYPESGWDRLRELFGKDEQQRISKDLANICKTAATAGIIGWVYGGIPAFIHAKQQYIEQSQAEIYHNRFDAVQSAHRAATRGFIRYGWRWGWRTAVFVTIFNTVNTSLNVYRNKDALSHFVIAGAVTGSLFRINVGLRGLVAGGIIGALLGTPVGGLLMAFQKYSGETVQERKQKDRKALHELKLEEWKGRLQVTEHLPEKIESSLQEDEPENDAKKIEALLNLPRNPSVIDKQDKD Predict reactive species
Full Name	chromosome 3 open reading frame 1
Calculated Molecular Weight	285 aa, 32 kDa
Observed Molecular Weight	32-35 kDa
GenBank Accession Number	BC012341
Gene Symbol	TIMMDC1
Gene ID (NCBI)	51300
RRID	AB_2879305
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen Affinity purified
UNIPROT ID	Q9NPL8
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Product Specific Protocols
IF protocol for TIMMDC1 antibody 23622-1-AP	Download protocol
WB protocol for TIMMDC1 antibody 23622-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Species	Application	Title
human	WB	Cancer Res miR-450a acts as a tumor suppressor in ovarian cancer by regulating energy metabolism. Authors - Bruna Rodrigues Muys View Article
human	WB	Immunobiology METTL3 regulates ASMCs proliferation and M2 macrophage polarization via mediating the m6A methylation of TIMMDC1 in asthma Authors - Shihao Zhang View Article