Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 1 publications below |
| IHC | See 1 publications below |
Product Information
11972-2-AP targets TIPIN in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag2595 Product name: Recombinant human TIPIN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-301 aa of BC000870 Sequence: MLEPQENGVIDLPDYEHVEDETFPPFPPPASPERQDGEGTEPDEESGNGAPVPVPPKRTVKRNIPKLDAQRLISERGLPALRHVFDKAKFKGKGHEAEDLKMLIRHMEHWAHRLFPKLQFEDFIDRVEYLGSKKEVQTCLKRIRLDLPILHEDFVSNNDEVAENNEHDVTSTELDPFLTNLSESEMFASELSRSLTEEQQQRIERNKQLALERRQAKLLSNSQTLGNDMLMNTPRAHTVEEVNTDEDQKEESNGLNEDILDNPCNDAIANTLNEEETLLDQSFKNVQQQLDATSRNITEAR Predict reactive species |
| Full Name | TIMELESS interacting protein |
| Calculated Molecular Weight | 301 aa, 34 kDa |
| Observed Molecular Weight | 48 kDa |
| GenBank Accession Number | BC000870 |
| Gene Symbol | TIPIN |
| Gene ID (NCBI) | 54962 |
| RRID | AB_2205116 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BVW5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TIPIN, also named as TIMELESS-interacting protein, is a 301 amino acid protein, which belongs to the CSM3 family. TIPIN Plays an important role in the control of DNA replication and the maintenance of replication fork stability. TIPIN forms a complex with TIMELESS and this complex regulates DNA replication processes under both normal and stress conditions, stabilizes replication forks and influences both CHEK1 phosphorylation and the intra-S phase checkpoint in response to genotoxic stress. The calculated molecular weight of TIPIN is 34 kDa, but the result of WB is 55-60 kDa, which is similar to the result of publication.(PMID: 24830473)
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for TIPIN antibody 11972-2-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dis Markers Integrated Bioinformatic Analysis Identifies TIPIN as a Prognostic Biomarker in Hepatocellular Carcinoma. | ||
Nature Mechanism for local attenuation of DNA replication at double-strand breaks
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