TJAP1 Polyclonal antibody

TJAP1 Polyclonal Antibody for WB, ELISA

Cat No. 17734-1-AP

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

PILT, Tight junction-associated protein 1, TJAP 1, TJP4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

SKU:

Freight/Packing: -

Quantity

- +

Filter: AllWB

WB analysis of human skeletal muscle using 17734-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 17734-1-AP (TJAP1 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17734-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17734-1-AP (TJAP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis using 17734-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 17734-1-AP (TJAP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

Positive WB detected in

4T1 cells, A431 cells, human skeletal muscle tissue, HSC-T6 cells, HepG2 cells, L02 cells, U-251 cells

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:8000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

17734-1-AP targets TJAP1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag12174 Product name: Recombinant human TJAP1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC064401 Sequence: MTSAAPAKKPYRKAPPEHRELRLEIPGSRLEQEEPLTDAERMKLLQEENEELRRRLASATRRTEALERELEIGQDCLELELGQSREELDKFKDKFRRLQNSYTASQRTNQELEDKLHTLIKKAEMDRKTLDWEIVELTNKLLDAKNTINKLEELNERYRLDCNLAVQLLKCNKSHFRNHKFADLPCELQDMVRKHLHSGQEAASPGPAPSLAPGAVVPTSVIARVLEKPESLLLNSAQSGSAGRPLAEDVFVHVDMSEGVPGDPASPPAPGSPTPQPNGECHSLGTARGSPEEELPLPAFEKLNPYPTPSPPHPLYPGRRVIEFSEDKVRIPRNSPLPNCTYATRQAISLS Predict reactive species
Full Name	tight junction associated protein 1 (peripheral)
Calculated Molecular Weight	547 aa, 61 kDa
Observed Molecular Weight	86-95 kDa
GenBank Accession Number	BC064401
Gene Symbol	TJAP1
Gene ID (NCBI)	93643
RRID	AB_2240742
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q5JTD0
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.