Tested Applications
| Positive IHC detected in | human pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | MDCK cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
23731-1-AP targets TMEM231 in IHC, IF/ICC, ELISA applications and shows reactivity with human, canine samples.
| Tested Reactivity | human, canine |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag20669 Product name: Recombinant human TMEM231 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 47-316 aa of BC010609 Sequence: GFWLKRSSYEEQPTVRFQHQVLLVALLGPESDGFLAWSTFPAFNRLQGDRLRVPLVSTREEDRNQDGKTDMLHFKLELPLQSTEHVLGVQLILTFSYRLHRMATLVMQSMAFLQSSFPVPGSQLYVNGDLRLQQKQPLSCGGLDARYNISVINGTSPFAYDYDLTHIVAAYQERNVTTVLNDPNPIWLVGRAADAPFVINAIIRYPVEVISYQPGFWEMVKFAWVQYVSILLIFLWVFERIKIFVFQNQVVTTIPVTVTPRGDLCKEHLS Predict reactive species |
| Full Name | hypothetical protein FLJ22167 |
| Calculated Molecular Weight | 345 aa, 39 kDa |
| GenBank Accession Number | BC010609 |
| Gene Symbol | TMEM231 |
| Gene ID (NCBI) | 79583 |
| RRID | AB_2879312 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9H6L2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Transmembrane protein 231 (TMEM231) encodes a 36 kDa two-pass transmembrane protein that is part of the B9 complex and localizes to the base of the ciliary axoneme at the TZ. TMEM231 was first identified as a causative gene for MKS and participates in the formation of the diffusion barrier between the cilia and plasma membrane (PMID: 34912761, 37736303). Tmem231 mutant mice have developmental defects reminiscent of MKS patients, including corticomedullary kidney cysts, polydactyly and hepatic ductal plate malformation (PMID: 25869670).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for TMEM231 antibody 23731-1-AP | Download protocol |
| IF protocol for TMEM231 antibody 23731-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
